Plant pyrophosphorylases that are capable of producing UDP-sugars, key precursors for glycosylation reactions, include UDP-glucose pyrophosphorylases (A- and B-type), UDP-sugar pyrophosphorylase and UDP-N-acetylglucosamine pyrophosphorylase. Although not sharing significant homology at the amino acid sequence level, the proteins share a common structural blueprint. Their structures are characte...

Alkaline phosphatase has been coupled to Griffonia simplicifolia I B4 isolectin using a one-step glutaraldehyde conjugation procedure. This enzyme-lectin conjugate (AP-GS I-B4) has been used to specifically detect plastic-bound natural and synthetic glycoproteins bearing alpha-D-galactopyranosyl end groups. The extent of reactivity of the AP-GS I-B4 with the glycoproteins appears to be proporti...

Vacuoles are the stores for large amounts of stachyose [alphagal (1,6) alphagal (1,6) alphaglc (1,2) betafru] in tubers of Japanese artichoke (Stachys sieboldii). The uptake of stachyose by these vacuoles was examined and compared with that of sucrose. The uptake mechanisms of both sugars were quite similar. The kinetics showed a single saturable response to increasing external concentrations o...

masses of 31 kDa. The serological specificity of the purified lectin was specifically inhibited by siatic acids sialoglycoproteins, but not by neutral sugars, hexosamines, IV-acetylhexosamines, or asialoglycopreteins. A]though the N-terminal amino acid sequence of the 1ectin from T. gigas was identical to that frem American horseshoe crab (liphemin) by the same pllrification method and cross re...

The capsular polysaccharide of Diplococctw pneumoniae Type XII contains residues of D-glucose and D-galactose in a molar ratio of 2:l. The methylated polysaccharide yielded upon hydrolysis 2,3,4,6-tetraand 3,4,6-tri-O-methyl-D-glucose and 2,3,4,6-tetra-0-methyl-D-galactose as the only neutral methyl sugars. Periodate oxidation of the polysaccharide resulted in destruction of all neutral sugars ...

The monoclonal antibody (MCA) SucroTest is a commercially available kit (Midland Research Laboratories Inc) for the specific analysis of dextran in juices and sugar. The method is simple and quick and is based on the selective and specific reaction between the antibody and its antigen (in this case dextran). The resultant insoluble complex causes turbidity which is measured after a set time usi...

conclusions the impact of sugars and other factors on biofilm formation can vary depending on the type of bacteria present. results results showed that the presence of sugars has no effect on the bacterial growth rate; all three concentrations of sugars were hydrophilic and demonstrated a high affinity toward binding to the surfaces. background bacteria utilize various methods in order to live ...

Extra-large pore zeolites are a small subset (21) among the whole list of 253 available. The discovery new low-glycemic sugars is very attractive as healthy additives in food field. This case 6-kestose. In present case, it appears mixture aqueous solution together with sucrose, separation being necessary. For this, we have focused on using certain adequate sizes that allow this mixture, conside...