نتایج جستجو برای: targeting cassette
تعداد نتایج: 157564 فیلتر نتایج به سال:
By targeting the AlP binding conserved domain in three AlP binding cassette superfamily proteins (P-glycoprotein, multidrug resistance pro tein, and cystic fibrosis transmembrane regulator), we isolated the cDNA of a new AlP binding cassette superfamily that was specifically enhanced in a cisplatin-resistant human head and neck cancer KB cell line. A human clone homologous to rat cnnalicular mu...
To develop a portable point-of-care system based on biosensors for common infectious diseases such as urinary tract infection, the sensing process needs to be implemented within an enclosed fluidic system. On chip sample preparation of clinical samples remains a significant obstacle to achieving robust sensor performance. Herein AC electrokinetics is applied in an electrochemical biosensor cass...
Gene targeting in most of human somatic cell lines has been labor-intensive because of low homologous recombination efficiency. The development of an experimental system that permits a facile evaluation of gene targeting efficiency in human somatic cell lines is the first step towards the improvement of this technology and its application to a broad range of cell lines. In this study, we utiliz...
Conditional gene deletion in mice has contributed immensely to our understanding of many biological and biomedical processes. Despite an increasing awareness of nonprotein-coding functional elements within protein-coding transcripts, current gene-targeting approaches typically involve simultaneous ablation of noncoding elements within targeted protein-coding genes. The potential for protein-cod...
By targeting the ATP binding conserved domain in three ATP binding cassette superfamily proteins (P-glycoprotein, multidrug resistance protein, and cystic fibrosis transmembrane regulator), we isolated the cDNA of a new ATP binding cassette superfamily that was specifically enhanced in a cisplatin-resistant human head and neck cancer KB cell line. A human clone homologous to rat canalicular mul...
Targeting construct, genotyping, colony generation and analysis. We have isolated Nobox genomic clones from a 129S6/SvEv genomic library and generated a targeting construct (Fig. S1A). The Nobox targeting construct was electroporated into AB2.2 ES cells to mutate the wild type Nobox locus by homologous recombination as previously described(S1,S 2). The mutant Nobox allele replaces exons 2-8 wit...
By targeting the AlP binding conserved domain in three AlP binding cassette superfamily proteins (P-glycoprotein, multidrug resistance pro tein, and cystic fibrosis transmembrane regulator), we isolated the cDNA of a new AlP binding cassette superfamily that was specifically enhanced in a cisplatin-resistant human head and neck cancer KB cell line. A human clone homologous to rat cnnalicular mu...
Conditional gene manipulation in mice becomes a routine for genetic studies of mammalian gene functions. Additional site-specific recombinases such as FLP or phi31 provide one more level of gene manipulation flexibility. The recombination activity of the currently available FLP deleter mice remains low. We generated a new FLP deleter mouse line with the mouse codon-optimized FLPo gene in C57BJ/...
Problem statement. In accordance with the code of rules (SP 101.13330.2012), use fish protection devices a filter screen and flushing system applies to water intake facilities in various placement conditions, this regard, it is relevant expand possibilities using cassettes at shallow depths source.
 Results. A methodology has been developed substantiate filler parameters horizontal cassett...
Insertion of a transgene into a defined genomic locus in human embryonic stem cells (hESCs) is crucial in preventing random integration-induced insertional mutagenesis, and can possibly enable persistent transgene expression during hESC expansion and in their differentiated progenies. Here, we employed homologous recombination in hESCs to introduce heterospecific loxP sites into the AAVS1 locus...
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