A strategy for the site-specific attachment of 2-deoxy-2-fluorosugars to cysteine and dehydroalanine tagged proteins is reported. When combined with thionation of fluorosugars, such as the widely available (18)F probe 2-deoxy-2-[(18)F]fluoroglucose ([(18)F]FDG), this methodology allows fast and direct access to site-specific [(18)F]FDG-labelled proteins.

A large number of spores from fruiting bodies can lead to allergic reactions and other problems during the cultivation of edible mushrooms, including Pleurotus eryngii (DC.) Quél. A cultivar harboring a sporulation-deficient (sporeless) mutation would be useful for preventing these problems, but traditional breeding requires extensive time and labor. In this study, using a sporeless P. eryngii ...

We consider the position of a tagged particle in the one-dimensional asymmetric nearest-neighbor simple exclusion process. Each particle attempts to jump to the site to its right at rate p and to the site to its left at rate q. The jump is realized if the destination site is empty. We assume p > q. The initial distribution is the product measure with density 2, conditioned to have a particle at...

A bodipy probe was developed for site-specific labeling of tagged proteins inside live cells which displays a large spectral change upon covalent coupling to the designed peptide that contains two pairs of Arg-Cys.

The decoration of iron oxide nanoparticles with Ni2+ ions provided the superparamagnetic nanoparticles with a binding site for His-tagged proteins, allowing their selective binding and convenient separation from a multi-component solution with an appropriately applied magnetic field.