Numerous studies have shown the positive effects of rosmarinic acid on the nervous system. Rosmarinic acid as a herbal compound with anti-inflammatory effects can prevent the destructive effect of inflammation on the nervous system. Furthermore, various studies have emphasized the advantages of three dimensional (3D) culture over the two dimensional (2D) culture of cells. In this study, thermos...

BACKGROUND The antiphospholipid syndrome (APS), characterised by recurrent miscarriage and thrombosis, is a significant cause of morbidity and mortality. Domain I (DI) of human beta 2 glycoprotein I (beta2GPI) is thought to contain crucial antibody binding epitopes for antiphospholipid antibodies (aPL), which are critical to the pathogenesis of APS. Expressing this protein in bacteria could fac...

the objective of this research was to investigate the influence of the over-expression of recombinant interferon-g during high cell density cultivation on cellular characteristics of recombinant e. coli. batch and fed-batch culture techniques were employed to grow escherichia coli bl21 for production of human gamma-interferon in pet expression system. final cell densities in batch and fed-batch...

The objective of this research was to investigate the influence of the over-expression of recombinant interferon-g during high cell density cultivation on cellular characteristics of recombinant E. coli. Batch and fed-batch culture techniques were employed to grow Escherichia coli BL21 for production of human gamma-interferon in pET expression system. Final cell densities in batch and fed-batch...

Gastric adenocarcinoma is the second leading cause of death due to cancer in the world and in advanced stages the prognosis is poor with current therapies. Over-expression of HER-2/neu has been seen in several cancers such as gastric cancer and its expression is associated with poor prognosis. The aim of this study is to evaluate the over-expression of HER-2/neu in gastric biopsy samples of pat...

background: cholera toxin b subunit (ctb) has been extensively considered as an immunogenic and adjuvant protein, but its yield of expression is not satisfactory in many studies. the aim of this study was to compare the expression of native and mutant recombinant ctb (rctb) in pqe vector. methods: ctxb fragment from vibrio cholerae o1 atcc14035 containing the substitution of mutant ctxb for ami...

Background: Mesenchymal stem cells (MSCs) are ideal cells for cell and gene therapy. However, the low survival of MSCs after transplantation has limited their applications. We aimed to evaluate the expression of cytoprotective genes including NQO1, TXNRD1, HO-1, GCLC following the over expression of Nrf2 in umbilical cord-derived MSCs (UC-MSCs). Methods: 3-5 passages of UC-MSCs were cultured. ...

background : amelogenins are the major components of enamel matrix proteins. enamel matrix derivatives (emd) can be used in periodontal diseases to regenerate periodontal tissues. the main aim of this study was to evaluate ex-pression of full-length functional recombinant human amelogenin (rham) in iranian lizard leishmania (i.l.l.) as an alternative eukaryotic expression system. methods : huma...

Inducible expression systems in which T7 RNA polymerase transcribes coding sequences cloned under control of a T7lac promoter efficiently produce a wide variety of proteins in Escherichia coli. Investigation of factors that affect stability, growth, and induction of T7 expression strains in shaking vessels led to the recognition that sporadic, unintended induction of expression in complex media...