Trichoderma species isolated from water-damaged buildings were screened for toxicity by using boar sperm cells as indicator cells. The crude methanolic cell extract from Trichoderma harzianum strain ES39 inhibited the boar sperm cell motility at a low exposure concentration (50% effective concentration, 1 to 5 microg [dry weight] ml of extended boar semen(-1)). The same exposure concentration d...

Biological control of soilborne plant pathogens using beneficial fungi, such as the mycoparasite Trichoderma harzianum, offers the prospect of environmentally benign pest control. However, biocontrol organisms have their own natural enemies; for example the fungivorous nematode Aphelenchoides saprophilus preys on T. harzianum. A trophic cascade occurs when three or more trophic levels are prese...

Exposing a wild-type culture of Trichoderma harzianum to gamma irradiation induced two stable salt-tolerant mutants (Th50M6 and Th50M11). Under saline conditions, both mutants greatly surpassed their wild type strain in growth rate, sporulation and biological proficiency against Fusarium oxysporum, the causal agent of tomato wilt disease. Tolerant T. harzianum mutants detained a capability to g...

Members of genus Trichoderma are known worldwide for mycoparasitism. To gain a better insight into the organization and evolution of their genomes, we used an in silico approach to compare the occurrence, relative abundance and density of SSRs in Trichoderma atroviride, T. harzianum, T. reesei, and T. virens. Our analysis revealed that in all the four genome sequences studied, the occurrence, r...

AIM The main goal of the present research conducted to assess the in vitro activity of the nematophagous fungi Duddingtonia flagrans, Fusarium solani, Verticillium chlamidosporium, and Trichoderma harzianum. MATERIAL AND METHODS Four isolates of fungi including D. flagrans, F. solani, V. chlamidosporium and T. harzianum were used in this study. Horse faeces were used to provide the larvae sta...

Profiling the transcriptome that underlies biomass degradation by the fungus Trichoderma harzianum allows the identification of gene sequences with potential application in enzymatic hydrolysis processing. In the present study, the transcriptome of T. harzianum IOC-3844 was analyzed using RNA-seq technology. The sequencing generated 14.7 Gbp for downstream analyses. De novo assembly resulted in...

The antagonistic effect of Trichoderma harzianum on a range of seed-borne fungal pathogens of wheat (viz. Fusarium graminearum, Bipolaris sorokiniana, Aspergillus spp., and Penicillium spp.) was assessed. The potential of T. harzianum as a biocontrol agent was tested in vitro and under field conditions. Coculture of the pathogens and Trichoderma under laboratory conditions clearly showed domina...

The role of the Trichoderma harzianum endochitinase (Ech42) in mycoparasitism was studied by genetically manipulating the gene that encodes Ech42, ech42. We constructed several transgenic T. harzianum strains carrying multiple copies of ech42 and the corresponding gene disruptants. The level of extracellular endochitinase activity when T. harzianum was grown under inducing conditions increased ...

Nineteen isolates of Trichoderma viride and Trichoderma harzianum obtained from rhizosphere soil of plantation crops, forest soil and agricultural fields of North Bengal region were studied using RAPD and ITS-PCR. The genetic relatedness among eleven isolates of T. viride and eight isolates of T. harzianum were analyzed with six random primers. RAPD profiles showed genetic diversity among the i...

trichoderma species are among important antagonists of plant pathogenic fungi. the main purpose of this study was to evaluate the biocontrol potential of native trichoderma spp. isolates from sugar beet fields against phytophthora drechsleri and p. cryptogea, the causal agents of phytophthora root rot of sugar beet. during 2007–08, 85 isolates of trichoderma spp. from sugar beet fields in eight...