The biosynthesis of psilocybin has been investigated by feeding labelled precursors to Psilocybe cubensis. The following specifically labelled compounds were synthesized: psilocin-H, 4hydroxytryptamine-, N,N-dimethyltryptamine-C and --H, N-methyltryptamine-C-H and DLtryptophan-H. A number of other indoles were labelled by acid catalyzed exchange in tritiated water. The experimental data suggest...

1. The incorporation of 14C into the brain glycogen of conscious rabbits with labelled glucose, bicarbonate and glutamate as precursors has been studied. 2. Substantial incorporation from all these precursors was demonstrated after an interval of 5hr. from their injection. 3. With [14C]glucose maximal incorporation occurred at about 8hr. from the time of injection. 4. Hydrocortisone led to incr...

The biosynthesis of the cyanogenic glucosides, linamarin and prunasin, was investigated in linen-flax, peach and cherry-laurel shoots. It was shown that related 2-oximino acids, aldoximes, nitriles and 2-hydroxynitriles were generally good precursors of the aglycone moiety. Studies with double-labelled compounds confirmed the retention of the oximino nitrogen atom from 2-oximinoisovaleric acid ...

The mRNA molecules coding for three mouse immunoglobulin A-type light (L) chains (MOPC-104E Al, RPC-20 Al, MOPC-315 A2) programme the cell-free synthesis of precursors larger than the mature proteins. Radioactive amino acid-sequence analyses of each of the three precursors labelled with [3H]alanine, [3H]serine, [3H]glutamine, [3H]glutamic acid and [3H]threonine showed that an extra piece, at le...

hepatomegaly (Nievel, 1969). The animals were killed 18h after the last dose of phenobarbitone. The rate of protein synthesis was assessed in uivo in dual-labelled experiments with [3H]and [14C]-leucine as precursors. Liver subcellular fractions were obtained by zonal and isopycnic ultracentrifugation. Various plasma and liver proteins, fibrinogen and plasminogen were fractionated and purified ...