نتایج جستجو برای: sybr green i

تعداد نتایج: 1167642  

2016
Yuanbin Liu Ting Lei Zhiyu Liu Yanbin Kuang Jianxin Lyu Qi Wang

Epidermal growth factor receptor (EGFR) gene mutations occur in multiple human cancers; therefore, the detection of EGFR mutations could lead to early cancer diagnosis. This study describes a novel EGFR mutation detection technique. Compared to direct DNA sequencing detection methods, this method is based on allele-specific amplification (ASA), recombinase polymerase amplification (RPA), peptid...

2007
Haukur Gudnason Martin Dufva D.D. Bang Anders Wolff

The importance of real-time polymerase chain reaction (PCR) has increased steadily in clinical applications over the last decade. Many applications utilize SYBR Green I dye to follow the accumulation of amplicons in real time. SYBR Green I has, however, a number of limitations that include the inhibition of PCR, preferential binding to GC-rich sequences and effects on melting curve analysis. Al...

Journal: :Iranian journal of biotechnology 2015
Xueqin Rao Jie Sun

BACKGROUND Watermelon silver mottle virus (WSMoV), which belongs to the genus Tospovirus, causes significant loss in Cucurbitaceae plants. OBJECTIVES Development of a highly sensitive and reliable detection method for WSMoV. MATERIALS AND METHODS Recombinant plasmids for targeting the sequence of nucleocapsid protein gene of WSMoV were constructed. SYBR Green I real-time PCR was established...

Journal: :BioTechniques 2012
Mario Van Poucke Alex Van Zeveren Luc J Peelman

Since its first use, real-time quantitative PCR (qPCR) has evolved into a flexible, application-made method for the quantification and identification of nucleic acids (1-2). Depending on the application, most researchers choose between fluorescent nucleic acid binding dyes or labels that interact by fluorescence resonance energy transfer (FRET) as nucleic acid detection methods (2-3). Binding d...

Journal: :Chemical communications 2009
Yen-Hsiu Lin Wei-Lung Tseng

We developed a new homogeneous assay-using SYBR Green I and repeats of 20 C nucleotides-for the highly selective and sensitive detection of silver ions and silver nanoparticles.

Journal: :The Analyst 2014
Quang Huy Quach Bong Hyun Chung

A simple, rapid, and signal-on fluorescent assay was developed for activity analysis of DNA methyltransferase and for screening of its inhibitors based on a methylation-resistant endonuclease and SYBR Green I.

Journal: :Chemical communications 2012
Xiangyuan Ouyang Jinhua Liu Jishan Li Ronghua Yang

Combining a DNA intercalator, SYBR Green I, and enzyme-linkage reactions with carbon nanoparticles, a low-background biosensing platform for label-free and sensitive fluorescent assay of DNA methylation is reported.

Journal: :Nucleic acids research 2004
Kyoko Takatsu Toyokazu Yokomaku Shinya Kurata Takahiro Kanagawa

Fluorescence resonance energy transfer (FRET) is a simple procedure for detecting specific DNA sequences, and is therefore used in many fields. However, the cost is relatively high, because FRET-based methods usually require fluorescent probes. We have designed a cost-effective way of using FRET, and developed a novel approach for the genotyping of single nucleotide polymorphisms (SNPs) and all...

Journal: :Canadian journal of microbiology 2011
Jennifer Loveland-Curtze Vanya I Miteva Jean E Brenchley

Standardized procedures must be followed when characterizing, officially describing, and validly naming novel bacteria. For species descriptions, DNA-DNA hybridization still is needed for whole-genome comparisons between close relatives, but many established hybridization methods have drawbacks, such as requiring labeled or large amounts of DNA. We evaluated a new technique based on the spectro...

2014
Jin Woo Jang Ju Yeon Kim Jung Yoon Soo Young Yoon Chi Hyun Cho Eun Taek Han Seong Soo A. An Chae Seung Lim

A flow cytometric (FACS) detection method for Plasmodium falciparum cultures (P. falciparum) was developed using SYBR Green I and CD235A and compared against the Giemsa stained microscopic examination. The cultured P. falciparum were spiked into red blood cells (RBCs) to yield parasitemia, ranging from 0.01% to 22.0%. FACS analysis demonstrated a clear separation between P. falciparum infected ...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید