نتایج جستجو برای: sybr green

تعداد نتایج: 140135  

Journal: :Journal of microbiological methods 2005
Subbarao V Ravva Larry H Stanker

Sensitive real-time sequence detection methods based on two different chemistries were developed for Mycobacterium avium subsp. paratuberculosis (Map), the causative agent of Johne's disease in cattle. One is based on the detection of SYBR Green bound to PCR products and the second method is more specific, detecting the cleavage of a fluorogenic (TaqMan) probe bound to a target sequence during ...

Journal: :The new microbiologica 2011
Elisabetta Delibato Alfonsina Fiore Fabrizio Anniballi Bruna Auricchio Emma Filetici Leucio Orefice Marina Nadia Losio Dario De Medici

Food-borne diseases caused by Salmonella represent a worldwide public health problem. Salmonella must be absent in an established amount depending on the kind of the product and usually cultural methods have to be applied to evaluate the compliance of the products. ISO 6579:2002 in Europe and FSIS MLG 4.04.:2008 in the USA have usually been employed to detect Salmonella in meat, poultry and egg...

ژورنال: :مجله دانشگاه علوم پزشکی فسا 0
بابک ممنون babak mamnoon cellular and molecular research center, faculty of medicine, qazvin university of medical sciences, qazvin, iran.مرکز تحقیقات سلولی و مولکولی، دانشکده پزشکی، دانشگاه علوم پزشکی قزوین، قزوین، ایران. تقی ناصرپور فریور taghi naserpour farivar cellular and molecular research center, faculty of medicine, qazvin university of medical sciences, qazvin, iran.مرکز تحقیقات سلولی و مولکولی، دانشکده پزشکی، دانشگاه علوم پزشکی قزوین، قزوین، ایران. محسن کریمی ارزنانی mohsen karimi arzenani molecular medicine department, pasteur institute of iran, tehran, iran.بخش پزشکی مولکولی، انستیتوپاستور ایران، تهران، ایران.

زمینه و هدف: اینترفرون ها خانواده ای از سایتوکاین ها هستند که در پاسخ ایمنی به عفونت های ویروسی نقش اساسی دارند. در جریان تولید اینترفرون نوترکیب در میزبان بیولوژیک، قطعاتی از اسیدنوکلئیک میزبان وارد محصول می شود. به علت وجود محدودیت های روش های قبلی در تشخیص این آلودگی ها، هدف از این مطالعه، استفاده از روش مولکولی سریع و حساس real time pcr در این ناخالصی ها می باشد.   مواد و روش ها: ابتدا با ا...

Journal: :Journal of andrology 1994
D L Garner L A Johnson S T Yue B L Roth R P Haugland

A new membrane-permeant DNA stain, SYBR-14, was used in combination with propidium iodide (PI) to estimate the proportion of living sperm in bovine semen. The SYBR-14 stained living sperm while PI only stained degenerate cells that had lost their membrane integrity. Staining with SYBR-14 resulted in the nuclei of living sperm fluorescing bright green. Aliquots containing nearly all living bovin...

2016
Yuanbin Liu Ting Lei Zhiyu Liu Yanbin Kuang Jianxin Lyu Qi Wang

Epidermal growth factor receptor (EGFR) gene mutations occur in multiple human cancers; therefore, the detection of EGFR mutations could lead to early cancer diagnosis. This study describes a novel EGFR mutation detection technique. Compared to direct DNA sequencing detection methods, this method is based on allele-specific amplification (ASA), recombinase polymerase amplification (RPA), peptid...

Journal: :molecular and biochemical diagnosis (journal) 2014
seyed hossein mousavi fard shahin merat kiana shahzamani reza ghanbari neda yahoo

background:accumulative research is in progress to clarify clinical aspects of gbv-c. the possibility of interaction between hcv and gbv-c as well as its consequence on development of liver diseases is the most important clinical aspect which encourages researchers to develop a rapid and cost effective technique for simultaneous detection of both viruses.methods: in this study, a sybr green rea...

Journal: :Nanoscale 2012
Ping Gong Bihua Shi Pengfei Zhang Dehong Hu Mingbin Zheng Cuifang Zheng Duyang Gao Lintao Cai

This work presents a method to visualize the degradation of exogenous DNA in living cells using a novel type of activatable fluorescence imaging probe. Deoxyribonuclease (DNase)-activatable fluorescence probes (DFProbes) are composed of double strands deoxyribonucleic acid (dsDNA) which is labeled with fluorophore (ROX or Cy3) and quencher on the end of one of its strands, and stained with SYBR...

Journal: :Molecular vision 2000
D A Simpson S Feeney C Boyle A W Stitt

PURPOSE To determine whether continuous monitoring of SYBR Green I fluorescence provides a reliable and flexible method of quantitative RT-PCR. Our aims were (i) to test whether SYBR Green I analysis could quantify a wide range of known VEGF template concentrations, (ii) to apply this method in an experimental model, and (iii) to determine whether 20 existing primer pairs could be used to quant...

Journal: :Microbes and Environments 2021

Microbial cell counting provides essential information for the study of abundance profiles and biogeochemical interactions with surrounding environments. However, it often requires labor-intensive time-consuming processes, particularly subseafloor sediment samples, in which non-cell particles are abundant. We developed a rapid straightforward method staining microbial intracellular DNA by SYBR ...

Background: Watermelon silver mottle virus (WSMoV), which belongs to the genus Tospovirus, causes significant loss in Cucurbitaceae plants. Objectives: Development of a highly sensitive and reliable detection method for WSMoV. Materials and Methods: Recombinant plasmids for targeting the sequence of nucleocapsid protein gene of WSMoV were constructed. SYBR Green I real-time PCR was established...

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