In an attempt to further increase transgene expression levels in plants over and above the enhancement obtained with a 5' untranslated leader intron, three different maize introns were inserted at three different positions within the coding sequence of the luciferase reporter gene. Constructs were transformed into maize (Black Mexican Sweet) cells and protoplasts, and their activity determined....

The replication of shuttle vectors derived from Wheat Dwarf Virus, a monopartite geminivirus, was studied in cultured maize endosperm cells, and in the Black Mexican Sweet (BMS) maize cell line. Using in vivo labeling and DNA methylation analysis, we showed that replication was initiated within 24 hrs after transfection, and did not require cell division in both cell lines. Copy numbers of 30,0...

The objective of the present study was to investigate susceptibility maize hybrids natural mycotoxins contamination: aflatoxin total (AFLA), deoxynivalenol (DON), zearalenon (ZEA) and fumonisins (FB). Hybrids were grown during two production years (2019 2020) in locations. Mycotoxin contamination grains evaluated five sweet hybrids. Contamination level investigated first cluster mainly by below...

Sweet potato (Ipomoea batatas L.) is an important starch-producing crop used worldwide. However, few studies have been conducted on the energy efficient, cost benefit, and greenhouse gas (GHG) emissions of sweet production. To address this issue, data were collected using a questionnaire for face-to-face interviews 78 growers 74 reference (i.e., rice, maize, potato) in Guangdong province. Resul...

ABSTRACT A previously uncharacterized virus was isolated from fall-planted sweet corn (Zea mays L., Syngenta GSS 0966) leaves showing fine chlorotic streaks. Symptomatic plants were negative in enzyme-linked immunosorbent assay against many maize viruses, but reacted weakly with antisera to Sorghum stunt mosaic virus suggesting a distant relationship between the viruses. The virus was readily t...

Transposable elements (TEs) are capable of inducing heritable de novo genetic variation. The sequences capable of reactivation, and environmental factors that induce mobilization, remain poorly defined even in well-studied genomes such as maize. We treated maize tissue culture with the demethylating agent 5-aza-2-deoxcytidine and examined long-term tissue culture lines to discover silenced TEs ...

Plant viruses have been widely used as vectors for foreign gene expression and virus-induced gene silencing (VIGS). A limited number of viruses have been developed into viral vectors for the purposes of gene expression or VIGS in monocotyledonous plants, and among these, the tripartite viruses Brome mosaic virus and Cucumber mosaic virus have been shown to induce VIGS in maize (Zea mays). We de...

Abstract. Anastasia L, Kodrat EA, Victor H, Sanjaya A, Pinontoan R. 2022. Isolation and characterization of indigenous amylolytic enzyme-producing Aspergillus sp. from sweet-flavored tapai. Biodiversitas 23: 5580-5586. are able to produce abundant enzymes that may have a crucial role in producing the sweet flavor This study was aimed evaluate activity isolated widely known tapai Jember, East Ja...

Maize is a crucial global commodity, which used not only for food, but also as an alternative crop in biogas production and major energy-supply ingredient animal diets. However, climate change jeopardizing current maize due to its direct impact on weather instability water availability or indirect effects regional suitability loss. Hence, new areas sweet cultivation should be considered the fut...

In this opinion, the GMO Panelassessed the five-event stack maize MON87427xMON89034x1507xMON88017x59122 and its 25 subcombinations, independently of their origin. The GMO Panelhas previously assessed the five single events combined to produce this five-event stack maize and 11 subcombinations of these events and did not identify safety concerns. No new data on the single events or their previou...