The in vitro effect of polybrominated biphenyls (FireMaster BP-6) on adenylate cyclase [ATP pyrophosphate lyase (cyclizing), EC 4.6.1.1] activity in plasma membranes of rat lung alveoli was determined. Two fractions of plasma membranes, PM-I (approximate d = 1.13 g/cm3) and PM-II (approximate d = 1.16 g/cm3) were isolated under hypotonic homogenization and fractionation conditions (1mM NaHCO3, ...

The oxidative assimilation of carbon compounds by nonproliferating yeast cells has been studied by several workers. Winzler (1940) found that when Saccharomyces cerevisiae was allowed to oxidize sodium acetate only 66 per cent of the substrate was oxidized; the remainder was incorporated into the cell. Winzler and Baumberger (1938) found that when glucose was the substrate only 26.5 per cent of...

RNA molecules labelled in BHK 2I cells following infection with vesicular stomatitis virus in the presence of Actinomycin D and 32PO 4 were examined. A complex pattern was obtained when the RN A was centrifuged in sucrose gradients, at least five radioactive peaks being found. These had sedimentation coefficients in sucrose gradients in o-I M-sodium acetate, pH 5.o, of approx. 38 S, 28 S, 20 S,...

polyhydroxyalkanoates (phas) is known to be temporarily stored by microorganisms in activated sludge, especially in the anaerobic-aerobic processes. the main objective of this study was to investigate the effects of fatty acids (vfas) compounds coexistence in substrate of sbr on phas productivity of activated sludge. it has been found that addition of different concentrations of vfas results in...

Fatty acid biosynthesis from Na[1-(14)C]acetate was characterized in plastids isolated from primary roots of 7-day-old germinating pea (Pisum sativum L.) seeds. Fatty acid synthesis was maximum at 82 nanomoles per hour per milligram protein in the presence of 200 micromolar acetate, 0.5 millimolar each of NADH, NADPH, and coenzyme A, 6 millimolar each of ATP and MgCl(2), 1 millimolar each of Mn...

Solanum anomalum Thonn. ex Schumach. (family Solanaceae), an edible shrub whose fruits and leaves are used medicinally to treat diseases including diabetes was evaluated for effect on alpha amylase glucosidase enzymes in vivo. The leaf extract (70-210 mg/kg) fractions (hexane, dichloromethane, ethyl acetate, methanol, 140 of S. were vivo inhibitory using starch, sucrose maltose as substrates. A...

Sludge was granulated in a hydrogen-producing acidogenic reactor when operated at 26°C, pH 5.5 treating a sucrose-rich wastewater. The influence of hydraulic retention time (HRT) and sucrose concentration on hydrogen production by the acidogenic granular sludge was investigated at a constant loading rate of 25 g-sucrose/(l·day). Results show that the gas composition was not greatly influenced b...