Conventional carbohydrate histochemistry and the binding patterns of 21 lectins were analysed to characterise the glycoconjugate content in the components of the vomeronasal organ of the armadillo Chaetophractus villosus. The mucomicrovillous complex of the sensory epithelium bound most of the lectins studied. No reaction was observed with Con A, PSA, S-Con A and SBA, and the sustentacular cell...

To investigate access failure (AF) and stroke rates of aortic procedures performed with upper extremity (UEA), compare results open surgical vs. percutaneous UEA techniques closure devices. A physician initiated, multicentre, ambispective, observational registry (SUPERAXA - NCT04589962) was carried out patients undergoing requiring UEA, including transcatheter valve replacement, arch, thoraco-a...

Peroxidase (HRP) conjugates of Dolichos biflorus agglutinin (DBA), Arachis hypogaea agglutinin (PNA) and Ulex europaeus agglutinin I (UEA I) were used as specific molecular probes in order to describe histological patterns of carbohydrate moieties in glycoconjugates of gastric mucosa. Specimens of normal mucosa, metaplasias and carcinomas were fixed, embedded in paraffin and stained with lectin...

A major problem encountered when isolating human microvascular endothelium is the presence of contaminating cells such as fibroblasts that rapidly over-grow the endothelial cells. We describe here a simple, rapid technique for purifying endothelial cells derived from the microvasculature of neonatal foreskin and osteoarthritic and rheumatoid arthritic synovium. This technique is based on the se...

Up to now, histochemical investigations of the goblet cells found in cases of postoperative maxillary cysts (POMC) have yielded somewhat variable results, while examinations of glycoproteins have been restricted to only a few methods. Recently, lectins, which have specificity for various carbohydrate residues, have been applied for the detection of glycoproteins. However, as far as the author i...

PURPOSE To isolate, culture, and characterize goblet cells from the conjunctiva of rats. METHODS Conjunctival tissue was surgically removed from Sprague-Dawley rats. Goblet cells were then isolated from the nictitating membrane and fornix using explant cultures. Cells derived from the explants were grown and propagated in RPMI medium supplemented with 10% fetal bovine serum. They were charact...

We used fracture-label to establish ultrastructural localization of glycoproteins in cross-fractured nuclei of duodenal columnar and exocrine pancreatic cells. Mannose residues were detected in cell nuclei by labeling freeze-fractured tissues with concanavalin A-horseradish peroxidase X colloidal gold (Con A-HRP X CG) or direct concanavalin A X colloidal gold (Con A X CG); fucose residues were ...

By using histochemical methods, the binding pattern of various lectins in the epithelium of normal human appendix vermiformis was assessed. In addition to plant and invertebrate sugar receptors with nominal monosaccharide specificity for alpha-L-Fuc (UEA-I), alpha-D-Man and alpha-D-Gluc (Con A), alpha-D-GalNAc (DBA), D-GalNAc (SBA, HPA) beta-D-Gal (RCA-I) and D-Gal (VAA), a mammalian beta-galac...

Although studies have demonstrated that androgen withdrawal increases thymic size, molecular mechanisms underlying this expansion remain largely unknown. We show that decreased androgen signaling leads to enhanced immigration of bone marrow T-cell precursors, as manifested by both an early increase of early thymic progenitors (ETP) and improved uptake of adoptively transferred quantified precur...

[This article summarises the literature on child sex offender (‘CSO’) recidivism and sexual behaviour, in order to consider the implications for the rules used at common law and under the uniform Evidence Acts (‘UEAs’) for excluding propensity/tendency and coincidence/similar fact evidence in trials involving multiple complainants. By analysing two distinct lines of authority from courts of app...