Incubation of Bradyrhizobium japonicum with the cultured soybean cell line SB-1 resulted in the adhesion of the bacteria to the plant cells. An antiserum was raised against B. japonicum, and the 125I-labeled immunoglobulin fraction was used to quantitate the number of bacteria bound to the soybean cells. The measurement of 125I-labeled antibody binding correlated well with parallel assays by mi...

The role of natural killer (NK) cells in infection-induced liver fibrosis remains obscure. In this study, we elucidated the effect of NK cells on Schistosoma japonicum (S. japonicum) egg-induced liver fibrosis. Liver fibrosis was induced by infecting C57BL/6 mice with 18-20 cercariae of S. japonicum. Anti-ASGM1 antibody was used to deplete NK cells. Toll-like receptor 3 ligand, polyinosinic-pol...

We identified by cloning and DNA sequence analysis the phosphate regulatory gene phoB of Bradyrhizobium japonicum. The deduced gene product displayed pronounced similarity to the PhoB protein of Sinorhizobium meliloti (71.4% identical amino acids). Escherichia coli (50.2%) and other bacterial species. Insertion of a kanamycin resistance cassette into phoB led to impaired growth of the B. japoni...

Cirsium japonicum is a wild perennial herb that has been used as an anti-hemorrhagic, anti-hypertensive and uretic agent in traditional Chinese medicine. Recently, it was reported that C. japonicum inhibits the growth of implanted cancer cells. However, the molecular mechanisms underlying the anti-cancer properties of C. japonicum are not fully understood. In this study, we investigated the eff...

UNLABELLED PREMISE OF THE STUDY We developed 10 new primers for amplifying microsatellite loci for both Leucosceptrum japonicum and L. stellipilum to study the genetic structure of hybrid zones between these two species. • METHODS AND RESULTS We isolated DNA fragments containing microsatellites from an enriched library and designed the 10 primer pairs amplifying the fragments. The number o...

Difficulty in controlling human Schistosoma japonicum infection is partly attributed to the presence of non-human definitive hosts. Water buffaloes are a major reservoir for transmission of S. japonicum to humans in China. However, in the Philippines, reports based on microscopic examination of buffalo stool identified a low prevalence of S. japonicum, and mathematical models using these data c...

in order to evaluate the effects of biofertilizer on soybean (glycine max l.) seed vigor that produced under water deficit condition and related traits, an experiment was conducted in a factorial layout based of complete randomized block design with four replications at the research greenhouse of aboureihan campus- tehran university, iran. experimental treatments were include biofertilizer (see...

The cultivation of soybeans, especially where this species has not been grown in large areas, requires the determination optimal mineral nitrogen fertilization and seed inoculation with Bradyrhizobium japonicum. purpose study was to determine dose N treatments B. japonicum under field conditions southeastern Baltic region. objective achieve supply and/or japonicum: check-0 kg ha−1, 30 60 HiStic...

Schistosoma japonicum infection is believed to be endemic in 28 of the 80 provinces of The Philippines and the most recent data on schistosomiasis prevalence have shown considerable variability between provinces. In order to increase the efficient allocation of parasitic disease control resources in the country, we aimed to describe the small-scale spatial variation in S. japonicum prevalence a...