Bovine Bacillus anthracis isolates from Cameroon were genetically characterized. They showed a strong homogeneity, and they belong, together with strains from Chad, to cluster Aβ, which appears to be predominant in western Africa. However, one strain that belongs to a newly defined clade (D) and cluster (D1) is penicillin resistant and shows certain phenotypes typical of Bacillus cereus.

Cob(I)alamin adenosyltransferase (EC 2.5.1.17) was purified to homogeneity from extracts of a Pseudomonas denitrificans recombinant strain and sequenced at its N terminus. It is a homodimer (each unit with an Mr of 28,000) encoded by cobO. The enzyme adenosylated all of the corrinoids isolated from this microorganism but did not adenosylate cobyrinic acid.

An extracellular protease-producing actinomycete, Streptomyces nogalator strain Ac 80, was isolated from soil. Mutants of the strain designated as Mt C producing protease of higher (3.0 mg tyrosine liberated/min/mg protein) and Mt L of lower (0.9 mg tyrosine liberated/min/mg protein) specific activity as compared to that of the wild type (1.8 mg tyrosine liberated/min/mg protein) were selected....

Time-dependent creep analysis is presented for the calculation of stresses and displacements of axisymmetric thick-walled cylindrical pressure vessels made of functionally graded material (FGM). For the purpose of time-dependent stress analysis in an FGM pressure vessel, material creep behavior and the solutions of the stresses at a time equal to zero (i.e. the initial stress state) are needed....

In this research, the analytical and numerical investigation of a cylindrical shell made of functionally graded materials (FGMs) reinforced by laminated composite subjected to internal pressure is presented. Using the infinitesimal theory of elasticity, the analytical solution of stress and strain in vessels made of FGMs is studied first. It is assumed that the elasticity modulus follows a powe...

The major carotenoid pigment of a psychrotrophic Micrococcus roseus strain was purified to homogeneity from methanol extracts of dried cells by reverse-phase liquid chromatography and was designated P-3. On the basis of the UV-visible, infrared, mass, and 'H nuclear magnetic resonance spectra of P-3, it was identified as bisdehydro-,-carotene-2-carboxylic acid. The pigment interacted with synth...

enzyme was purified to homogeneity as indicated by a single band in disc-gel electrophoretic columns and single peaks with column chromatography and ultracentrifugation from the wild-type PRL-R3 strain, induced with L-fucose and two constitutive mutants, 502 and 510. The ratios of the activities of this isomerase on Darabinose and L-fucose remained constant throughout all purifications. The app...