نتایج جستجو برای: stimulated by his environment

تعداد نتایج: 7350401  

Journal: :History of European Ideas 2016

Journal: :Proceedings of the Royal Society of Medicine 1953

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه شیراز - دانشکده علوم انسانی 1391

tennessee williams (1911-1983), the modern american dramatist, had his own unique school of dramaturgy. the dramas which he depicted are populated by characters who are lonely, desperate, in anxiety, alienated and in one word lost. they face challenges which they may overcome or not, through the choices they make. all these moods and conditions are clearly seen and explained in the theory of ex...

Journal: :Acta crystallographica. Section F, Structural biology and crystallization communications 2007
Akari Shinohara Mayuko Sakuma Toshiharu Yakushi Seiji Kojima Keiichi Namba Michio Homma Katsumi Imada

The polar flagellum of Vibrio alginolyticus is rotated by the sodium motor. The stator unit of the sodium motor consists of four different proteins: PomA, PomB, MotX and MotY. MotX and MotY, which are unique components of the sodium motor, form the T-ring structure attached to the LP ring in the periplasmic space. MotY has a putative peptidoglycan-binding motif in its C-terminal region and MotX...

2011
Ryan Choi Angela Kelley David Leibly Stephen Nakazawa Hewitt Alberto Napuli Wesley Van Voorhis

The recombinant expression of soluble proteins in Escherichia coli continues to be a major bottleneck in structural genomics. The establishment of reliable protocols for the performance of small-scale expression and solubility testing is an essential component of structural genomic pipelines. The SSGCID Protein Production Group at the University of Washington (UW-PPG) has developed a high-throu...

Journal: :BMC Biotechnology 2003
Christoph Scheich Volker Sievert Konrad Büssow

BACKGROUND Functional Genomics, the systematic characterisation of the functions of an organism's genes, includes the study of the gene products, the proteins. Such studies require methods to express and purify these proteins in a parallel, time and cost effective manner. RESULTS We developed a method for parallel expression and purification of recombinant proteins with a hexahistidine tag (H...

Journal: :Nanoscale 2013
Dris Ihiawakrim Ovidiu Ersen Frédéric Melin Petra Hellwig Izabela Janowska Dominique Begin Walid Baaziz Sylvie Begin-Colin Cuong Pham-Huu Rachid Baati

A practically simple top-down process for the exfoliation of graphene (GN) and few-layer graphene (FLG) from graphite is described. We have discovered that a biocompatible amphiphilic pyrene-based hexahistidine peptide is able to exfoliate, functionalize, and dissolve few layer graphene flakes in pure water under exceptionally mild, sustainable and virtually innocuous low intensity cavitation c...

Journal: :Protein science : a publication of the Protein Society 2005
Sreedevi Nallamsetty Brian P Austin Kerri J Penrose David S Waugh

Many proteins that accumulate in the form of insoluble aggregates when they are overproduced in Escherichia coli can be rendered soluble by fusing them to E. coli maltose binding protein (MBP), and this will often enable them to fold in to their biologically active conformations. Yet, although it is an excellent solubility enhancer, MBP is not a particularly good affinity tag for protein purifi...

Journal: :Applied and environmental microbiology 2005
Hyeon-Su Ro Sun Ok Jung Byung Hoon Kho Hyung Pyo Hong Jae Sung Lee Yong-Beom Shin Min Gon Kim Bong Hyun Chung

A surface plasmon resonance imaging-based Ni(2+)-iminodiacetic acid-coated gold chip system was developed to enable specific detection of a hexahistidine-tagged recombinant protein in crude extracts or in column chromatography fractions. This system is especially advantageous for high-throughput analysis of multiple proteins.

Journal: :Journal of the American Chemical Society 2008
Kalina Peneva Gueorgui Mihov Andreas Herrmann Nawid Zarrabi Michael Börsch Thomas M Duncan Klaus Müllen

Fluorescent probes are essential for the exploration of protein function, detection of molecular interactions, and conformational changes. The nitrilotriacetic acid derivatives of different chromophores were successfully used for site-selective noncovalent fluorescence labeling of histidine-tagged proteins. All of them, however, suffer from the same drawback--loss of the fluorescence upon bindi...

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