Stevia rebaudiana, a perennial herb from the Asteraceae family, is known to the scientific world for its sweetness and steviol glycosides (SGs). SGs are the secondary metabolites responsible for the sweetness of Stevia. They are synthesized by SG biosynthesis pathway operating in the leaves. Most of the genes encoding the enzymes of this pathway have been cloned and characterized from Stevia. O...

The role of mitogen-activated protein kinase (MAPK) signaling pathways in cell growth and differentiation has been well established. The present study aimed to investigate the anti-proliferative effect of stevioside on human colon cancer HT-29 cells. Additionally, the effect of stevioside on cell cycle arrest and MAPK signaling pathways in HT-29 cells was explored. Stevioside was observed to si...

Stevia rebaudiana Bertoni, member of Asteraceae family, has bio-active compounds stevioside and rebaudioside which taste about 300 times sweeter than sucrose. It regulates blood sugar, prevents hypertension and tooth decay as well as used in treatment of skin disorders having high medicinal values, and hence there is a need for generating the plant on large scale. We have developed an efficient...

The structure activity relationship between the C₁₆-C₁₇ methylene double bond on the aglycone of steviol glycosides and the corresponding impact on their sweet taste has been reported here for the first time. It has been observed that converting stevioside and rebaudioside A to their corresponding ketones by switching the doubly bonded methylene on C-17 for a ketone group actually removes the s...

Steviol glycosides are highly sweet diterpene glycosides found in the Paragyan shrub, Stevia rebaudiana Bertoni. Steviol glycosides, mainly stevioside and rebaudioside, principal components of Stevia rebaudiana leaf, has become well known for their sweetness and are used as non-caloric sweeteners in several countries due to their nutritional and pharmacological benefits. Hence, craving for swee...

Stevioside is a naturally occurring diterpenoid glycoside in Stevia rebaudiana Bertoni. The title compound, C38H60O18·4CH3OH, crystallized as its methanol tetrasolvate. Stevioside consists of an aglycone steviol (a tetra-cyclic diterpene in which the four-fused-ring system consists of three six-membered rings and one five-membered ring) and a sugar part (three glucose units). A weak intra-molec...

The genomic DNA polymorphism and phytochemical variation of Stevia rebaudiana Bertoni was investigated by RAPD-PCR and HPLC analysis. For initial PCR screening, about hundred oligo-nucleotide primers were used to amplify the genomic DNA from three accessions of S. rebaudiana (L1 to L3). The concentration of stevioside content was quantified by using HPLC analysis. A total of 9 primers were fina...

Stevioside and its major metabolite, steviol, have been reported to affect ion transport in many types of tissues, such as the kidney, pancreas, and intestine. The effect of stevioside, steviol, and its analogs on intestinal Cl(-) secretion was investigated in a human T84 epithelial cell line. Short-circuit current measurements showed that steviol and analogs isosteviol, dihydroisosteviol, and ...

Stevia rebaudiana is a plant with high sweetening capacity due to its content of glycosides, mainly stevioside and rebaudioside A. Several techniques have been used to determine the concentrations of glycosides in Stevia, although an HPLC method is recommended by the FAO/WHO-JECFA. Varieties of Stevia have been recently grown in Mexico, with no previous report of glycosides by a validated metho...

Flavonoids and phenolic acids play roles in grapevine defence against pathogens causing trunk diseases (GTDs). Rutin is a major flavonoid vegetative organs of the grapevines, this compound, unlike other flavonoids, non-toxic non-oxidizable. was assayed vitro vivo two Botryosphaeriaceae taxa. The limited bioavailability compound circumvented by conjugation with stevioside, glycoside obtained fro...