IgG antibodies directed against Staphylococcus aureus were examined by Western blotting in sera from 15 healthy individuals isolated for a year in Antarctica. Sera reacted with many staphylococcal antigens in whole-cell extracts and individuals showed unique and unchanging blot profiles. The IgG and IgM profiles of patients with deep-seated staphylococcal infections were also examined by Wester...

We describe the complete sequence of the 15.9-kb staphylococcal pathogenicity island 3 encoding staphylococcal enterotoxin serotypes B, K, and Q. The island, which meets the generally accepted definition of pathogenicity islands, contains 24 open reading frames potentially encoding proteins of more than 50 amino acids, including an apparently functional integrase. The element is bordered by two...

Background Elevated IgE antibodies to Staphylococcal toxins (superantigens) are related to asthma severity in a few studies. We have investigated this hypothesis in an adult asthmatic population from the Clinical Immunology outpatient service of an University Hospital. Our objectives were: to detect the presence and the degree of IgEmediated sensitization to staphylococcal toxins in vitro in as...

BACKGROUND Pre-operative antibody levels have been shown to be inversely related to development of post-operative complications. Staphylococcal infection is a major source of morbidity following surgery. METHODS We examined the variability of anti-staphylococcal antibody levels across a group of healthy volunteers and compared this with patients scheduled to undergo cardiac surgery. RESULTS...

Staphylococcal pyrogenic exotoxin (PE) types A and B were tested for their role in production of a scarlatiniform rash. The PEs elicited minimal skin reactions after intracutaneous injection into animals not presensitized to the toxins. In contrast, erythematous injection into animals not presensitized to the toxins. In contrast, erythematous and edematous rashes were produced after administrat...

Staphylococcal food poisoning is one of the most prevalent causes foodborne intoxication worldwide. Sandwiches and desserts are susceptible to contamination by S. aureus due high proportion manual work during their production. Our study aimed evaluate impact storage conditions on staphylococcal enterotoxin production in sandwiches buttercream puffs. Foods were inoculated with different strains ...

Staphylococcal food poisoning represents the most prevalent foodborne intoxication worldwide. It is caused by oral intake of enterotoxins preformed by Staphylococcus aureus in food. The relevance of newly described enterotoxins in outbreaks of staphylococcal food poisoning is controversially discussed. Although the staphylococcal enterotoxins SEG, SEI, SEM, SEN, and SEO elicit emesis in a monke...

BACKGROUND Rheumatoid arthritis (RA) is the most common chronic inflammatory disease. The staphylococcal superantigens are considered as the causative agent of RA disease. OBJECTIVES This study aimed to assess the presence of staphylococcal enterotoxin D in synovial fluid and blood of patients with RA. PATIENTS AND METHODS A total of 120 blood and SF samples of patients with RA were studied...