نتایج جستجو برای: ssr enrichment libraries

تعداد نتایج: 87836  

2013
Aline R. Martins Aluana G. Abreu Miklos M. Bajay Priscilla M. S. Villela Carlos E. A. Batista Mariza Monteiro Alessandro Alves-Pereira Glyn M. Figueira José B. Pinheiro Beatriz Appezzato-da-Glória Maria I. Zucchi

UNLABELLED PREMISE OF THE STUDY A new set of microsatellite or simple sequence repeat (SSR) markers were developed for Smilax brasiliensis, which is popularly known as sarsaparilla and used in folk medicine as a tonic, antirheumatic, and antisyphilitic. Smilax brasiliensis is sold in Brazilian pharmacies, and its origin and effectiveness are not subject to quality control. • METHODS AND RE...

2003
Jean Gougat Bernard Ferrari Lionel Sarran Claudine Planchenault Martine Poncelet Jeanne Maruani Richard Alonso Annie Cudennec Tiziano Croci Fabio Guagnini Katalin Urban-Szabo Jean-Pierre Martinolle Philippe Soubrié

2011
Anna Kiialainen Olof Karlberg Annika Ahlford Snaevar Sigurdsson Kerstin Lindblad-Toh Ann-Christine Syvänen

Targeted sequencing is a cost-efficient way to obtain answers to biological questions in many projects, but the choice of the enrichment method to use can be difficult. In this study we compared two hybridization methods for target enrichment for massively parallel sequencing and single nucleotide polymorphism (SNP) discovery, namely Nimblegen sequence capture arrays and the SureSelect liquid-b...

Journal: :Applied and environmental microbiology 2001
P Entcheva W Liebl A Johann T Hartsch W R Streit

Enrichment cultures of microbial consortia enable the diverse metabolic and catabolic activities of these populations to be studied on a molecular level and to be explored as potential sources for biotechnology processes. We have used a combined approach of enrichment culture and direct cloning to construct cosmid libraries with large (>30-kb) inserts from microbial consortia. Enrichment cultur...

Journal: :Nucleic acids research 1988
B Taidi-Laskowski D Tyan S M Honigberg C R Radding F C Grumet

RecA protein-coated probe has been utilized to enrich genomic digests for desired genes in order to facilitate cloning from genomic libraries. Using a previously cloned HLA-B27 gene as the recA-coated enrichment probe, we obtained a mean 108x increase in the ratio of specific to nonspecific plaques in lambda libraries screened for B27 variant alleles of estimated 99% homology to the probe. Clas...

2011
Gaurav Sablok Chun Luo Wan Sin Lee Farzana Rahman Tatiana V. Tatarinova Jennifer Ann Harikrishna Zhengrong Luo

We present here a systematic analysis of the Diospyros kaki expressed sequence tags (ESTs) generated from development stage-specific libraries. A total of 2,529 putative tentative unigenes were identified in the MF library whereas the OYF library displayed 3,775 tentative unigenes. Among the two cDNA libraries, 325 EST-Simple sequence repeats (SSRs) in 296 putative unigenes were detected in the...

Journal: :Journal of nematology 2009
Renée S Arias Salliana R Stetina Jennifer L Tonos Jodi A Scheffler Brian E Scheffler

Rotylenchulus reniformis is the predominant parasitic nematode of cotton in the Mid South area of the United States. Although variable levels of infection and morphological differences have been reported for this nematode, genetic variability has been more elusive. We developed microsatellite-enriched libraries for R. reniformis, produced 1152 clones, assembled 694 contigs, detected 783 simple ...

2017
Sihuan Zhang Han Xu Xinfeng Liu Qing Yang Chuanying Pan Chuzhao Lei Ruihua Dang Hong Chen Xianyong Lan

In practical production, almost all rams and about 50% of ewes are used to fatten. Researchers have proved that ewe ovariectomy could improve the productivity significantly, but the specific molecular mechanism is still unknown. In this study, five independent cDNA libraries (three and two from ovariectomized and normal ewe longissimus dorsi samples, respectively) were constructed to thoroughly...

2014
Yasmina El Bahloul Nicolas Dauchot Ikrame Machtoun Fatima Gaboun Pierre Van Cutsem

UNLABELLED PREMISE OF THE STUDY Microsatellite loci were developed for the Moroccan endemic endangered species Argania spinosa with a combination of a typical library enrichment procedure and a 454 GS FLX Titanium-based high-throughput sequencing approach. • METHODS AND RESULTS A genomic DNA library was enriched and further screened using (GA)15, (GTA)8, and (TTC)8 biotin-labeled probes co...

2012
K. R. Gedye J. L. Gonzalez-Hernandez V. Owens A. Boe

Prairie cordgrass (Spartina pectinata Bosc ex Link) is an indigenous, perennial grass of North America that is being developed into a cellulosic biomass crop suitable for biofuel production. Limited research has been performed into the breeding of prairie cordgrass; this research details an initial investigation into the development of a breeding program for this species. Genomic libraries enri...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید