Attractant and repellent signaling conformers of the dual-signaling phototaxis receptor sensory rhodopsin I and its transducer subunit (SRI-HtrI) have recently been distinguished experimentally by the opposite connection of their retinylidene protonated Schiff bases to the outwardly located periplasmic side and inwardly located cytoplasmic side. Here we show that the pK(a) of the outwardly loca...

Halophilic archaea, such as Halobacterium salinarum and Natronobacterium pharaonis, alter their swimming behavior by phototaxis responses to changes in light intensity and color using visual pigment-like sensory rhodopsins (SRs). In N. pharaonis, SRII (NpSRII) mediates photorepellent responses through its transducer protein, NpHtrII. Here we report the expression of fusions of NpSRII and NpHtrI...

Proteomics techniques for analysing the redox status of individual proteins in complex mixtures tend to identify the same proteins due to their high abundance. We describe here an array-based technique to identify proteins undergoing glutathionylation and apply it to the secretome and the proteome of human monocytic cells. The method is based on incorporation of biotinylated glutathione (GSH) i...

Barium stars are chemically peculiar that exhibit enhancement of s-process elements. Chemical abundance analysis barium can provide crucial clues for the study chemical evolution Galaxy. The Large Sky Area Multi-Object Fiber Spectroscopic Telescope (LAMOST) has released more than 6 million low-resolution spectra FGK-type by Data Release 9 (DR9), which significantly increase sample size stars. I...

Increasing quantity and quality of roads are pathways to sustainable road infrastructure development. Understanding quantity-quality relations sustainability is critically required for strategic decision making. However, few knowledge available about assessing from a perspective spatial disparities. Here, we developed Spatial Trade-Off Relation (STOR) model trade-off at 42,425 blocks in Western...