Regulation of alternative splicing is controlled by pre-mRNA sequences (cis-elements) and trans-acting protein factors that bind them. The combinatorial interactions of multiple protein factors with the cis-elements surrounding a given alternative splicing event lead to an integrated splicing decision. The mechanism of multifactorial splicing regulation is poorly understood. Using a splicing-se...

telomerase and systems controlling their activity have been of great attention. there are controversies regarding the role of the alternative splicing forms of the human telomerase reverse transcriptase (htert), the catalytic subunit of telomerase. therefore, the correlation between telomerase enzyme activity, the abundance of alternatively spliced variants of htert and doubling time of a serie...

Submit Manuscript | http://medcraveonline.com Abbreviations: NMD: Nonsense-Mediated mRNA Decay; ISE: Intronic Splicing Enhancer; ESE: Exonic Splicing Enhancer; ISS: Intronic Splicing Silencer; ESS: Exonic Splicing Silencer; PTC: Premature Termination Codon; ASC: Adenosquamous Carcinoma; NAS: Nonsense-associated Altered Splicing; NASRE: NonsenseAssociated Altered Splicing of a Remote Exon; ASO: ...

Two recent publications illuminate the evolution of alternative splicing, showing that a SR (serine-arginine-rich) protein that regulates alternative splicing in multicellular organisms is also found in a unicellular organism without alternative splicing, in which it can assist in the splicing of weak introns. Moreover, insertion of SR proteins into an organism lacking such proteins can restore...

Yue, B-G. 2000. Regulation of adenovirus alternative pre-mRNA splicing. Functional characterization of exonic and intronic splicing enhancer elements. Acta Universitatis Upsaliensis. Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine 926. 55pp. Uppsala. ISBN 91-554-4712-0 Pre-mRNA splicing and alternative pre-mRNA splicing are key regulatory steps controlling gene exp...

Numerous studies report splicing alterations in a multitude of cancers by using gene-by-gene analysis. However, understanding of the role of alternative splicing in cancer is now reaching a new level, thanks to the use of novel technologies allowing the analysis of splicing at a large-scale level. Genome-wide analyses of alternative splicing indicate that splicing alterations can affect the pro...

Nucleic acid therapeutics have demonstrated an impressive acceleration in recent years. They work through multiple mechanisms of action, including the downregulation gene expression and modulation RNA splicing. While several drugs based on former mechanism been approved, few target latter, despite promise splicing modulation. To improve our ability to discover novel splicing-modulating therapie...

SR proteins are essential splicing factors required for constitutive splicing and function as key regulators of alternative RNA splicing. We have shown that SR proteins purified from late adenovirus-infected cells (SR-Ad) are functionally inactivated as splicing enhancer or splicing repressor proteins by a virus-induced partial de-phosphorylation. Here, we show that SR proteins purified from la...

Pre-mRNA splicing is mediated by interactions of the Core Spliceosome and an array of accessory RNA binding proteins with cis-sequence elements. Splicing is a major regulatory component in higher eukaryotes. Disruptions in splicing are a major contributor to human disease. One in three hereditary disease alleles are believed to cause aberrant splicing. Hereditary disease alleles can alter splic...

Alternative splicing is the primary source of proteome complexity in metazoans and its regulation shapes the proteome in response to shifting physiological requirements. We developed a bichromatic splicing reporter that uses a peculiar feature of some fluorescent protein coding regions to express two different fluorescent proteins from a single alternative splicing event. The mutually exclusive...