brucellosis, caused by brucella spp., is an important zoonotic disease that causes abortion and infertility in cattle and undulant fever in humans. various studies have examined cell-free native and recombinant proteins as candidate protective antigens in animal models. among brucella immunogenes, antigen based on ribosomal preparation has been widely investigated. in this study, the immunogeni...

The aim of this study was to investigate the effect zinc (Zn) deficiency on growth and grain yield wheat with different allele statuses Gpc-B1 gene. For research, common wild emmer (Triticum turgidum ssp. dicoccoides (Koern. ex Asch. &Graebn.) Schweinf.), bread aestivum L. cv. Festivalnaya), two intogressive lines were used. T. introgressive line 15-7-1 carry a functional gene, while Festiv...

The gene encoding the spike glycoprotein (S) of bovine enteric coronavirus (BECV) was cloned and its complete sequence of 4092 nucleotides was determined. This sequence contained a single long open reading frame with a coding capacity of 1363 amino acids (Mr 150,747). The predicted protein had 19 N-glycosylation sites. A signal sequence comprising 17 amino acids was observed starting from the f...

Shared host cells can serve as melting pots for viral genomes, giving many phylogenies a web-like appearance due to horizontal gene transfer. However, not all virus families exhibit web-like phylogenies. Microviruses form three distinct clades, represented by φX174, G4, and α3. Here, we investigate protein-based barriers to horizontal gene transfer between clades. We transferred gene G, which e...

BACKGROUND: Management of nutrients, especially nitrogen, in order to have economic production, maintain sustainable agriculture and provide food security, is considered to have an important priority. OBJECTIVES: Current study was conducted to evaluate effect of different level of nitrogen fertilizer and vermicompost on quantitative and qualitative traits of w...

alterations in the expression of micrornas (mirnas) have been proposed to play a role in the pathogenesis of acute lymphoblastic leukemia (all) and chronic lymphocytic leukemia (cll). dicer is one of the main regulators of mirna biogenesis, and deregulation of its expression has been indicated as a possible cause of mirna alterations observed in various cancers. our aim was to analyze the expre...

SARS-CoV-2 requires serine protease, transmembrane protease 2 (TMPRSS2), and cysteine proteases, cathepsins B, L (CTSB/L) for entry into host cells. These proteases activate the spike protein enable entry. We herein performed genomic-guided gene set enrichment analysis (GSEA) to identify upstream regulatory elements altering expression of TMPRSS2 CTSB/L. Further, medicinal compounds were identi...

Diversity among the pathogenic strains of Theileria equi (T. equi), a major agent of equine piroplasmosis, can affect the appropriate detection of parasite and host immunization. Production of recombinant surface proteins from an infected horse in natural endemic area provides a reliable tool for immunodiagnosis of parasite. Regarding this, the present study was targeted toward the cloning, exp...

Abstract In order to study the inheritance of grain yield and its components in bread wheat, two cultivars, Karchyya(drought and salinity tolerant) and Gaspard (sensitive to drought and salinity stress) were crossed.Parents together with F1, F2, F3, BC1and BC2 generations were evaluated using a randomized complete block design with three replications in 2008-2009 growing season.Grain weight per...

SARS-CoV-2, causing the COVID-19 pandemic, has claimed millions of lives worldwide. SARS-CoV-2 a high mutation rate in its genome, resulting thousands variants. The success is attributed to S gene, which encodes spike protein that interacts directly with hACE2. Mutations this gene are known increase transmission and ability escape immune system virus. This study focused on analyzing nucleotide ...