Background: Phototherapies based on sunlight, infrared, ultraviolet, visible, and laser-based treatments present advantages like high curative effects, small invasion, negligible adverse reactions in cancer treatment. We aimed to explore the potential therapeutic effects of blue light emitting diode (LED) human hepatoma cells decipher underlying cellular molecular mechanisms. Methods:...

PURPOSE To isolate precursor cells derived from rabbit corneal endothelium (CE) and to use them for the treatment of CE deficiency in a rabbit model. METHODS A sphere-forming assay was performed to isolate precursor cells from rabbit CE. Immunocytochemistry was used to examine marker expressions of neural and mesenchymal cells in the sphere colonies and their progenies. The pump function of t...

Sphere formation assay is widely used in selection and enrichment of normal stem cells or cancer stem cells (CSCs), also known as tumor initiating cells (TICs), based on their ability to grow in serum-free suspension culture for clonal proliferation. However, there is no standardized parameter to accurately score the spheres, which should be reflected by both the number and size of the spheres....

Increased serum concentrations of tumor necrosis factor α (TNFα) and transforming growth factor β-1 (TGFβ-1) in the blood of patients with pancreatic cancer (PC) have previously been demonstrated. In addition, exogenous exposure to these cytokines promotes various cancer cell invasive and cancer stem cell (CSC) phenotypes. However, their importance in pancreatic CSCs remains elusive. In the pre...

Cancer stem cells (CSCs) are a small proportion of tumor cells that may be responsible for tumor metastasis and recurrence. Our recent research indicated that longikaurin A (LK-A) exhibited anti-tumor activity in nasopharyngeal carcinoma (NPC) both in vitro and in vivo. Here, we further investigated whether LK-A could suppress the stemness of NPC cells. Sphere formation assay was used to assess...

background and objectives:the plants of genus artemisia (asteraceae) have been conventionally used for prevention and medication of a number of ailments. in the present research, ten extracts with different polarities from aerial parts of two artemisia species, a. kopetdaghensis and a. turcomanica were evaluated for their potential anti-malarial properties. methods: the plant materials were ext...

BACKGROUND Despite the use of aggressive therapy, survival rates among high-risk neuroblastoma (NB) patients remain poor. Cancer stem cells (CSCs) are considered to be critically involved in the recurrence and metastasis of NB and are isolated as NB spheres. METHODS The gene expression profiling of adherent (control) and sphere-forming primary NB cells was conducted using a gene expression mi...

Considerable evidence suggests that many malignancies are driven by a cellular compartment that displays stem cell properties. Cancer stem-like cells (CSCs) can be identified by expression of cell surface markers or enzymatic activity, but these methods are limited by phenotypic heterogeneity and plasticity of CSCs. An alternative phenotypic methodology based on in-vitro sphere formation has be...

along with improvement and expansion if computational communications and increasing access of various peoples to the internet and virtual social networks, a lot of theoretical endeavors have been spent for studying the internet and its political and social effects. during these attempts, the concept of public sphere in the framework of habermas's theory has a significant status. accordingl...

Abstract While a large fraction of the stars are in multiple systems, our understanding processes leading to formation these systems is still inadequate. Given theoretical uncertainties, observation plays basic role. Here we discuss contribution high contrast imaging, and more specifically SPHERE instrument at ESO Very Large Telescope, this area. nicely complements other instruments such as Gai...