Sperm vitrification has been recently developed, but fertility trials have not performed yet in equine species. In this study, a new warming technique for vitrified donkey semen was developed and the uterine inflammatory response were compared to conventional freezing. Experiment 1, sperm straws warmed 3 ml of extender or water bath at: 37 °C/30 s; 43 °C/10 60 °C/5 s. motility, plasma acrosome ...

OBJECTIVE To evaluate the integrity of genomic imprinting in oocytes vitrified at the germinal vesicle (GV) stage and in vitro matured (IVM) after thawing. DESIGN Clinical research and application. SETTING University-based fertility center. PATIENT(S) Immature oocytes were donated for research by patients who were included in an intracytoplasmic sperm injection program. INTERVENTION(S) ...

Background: Nowadays, cryoprotectant-free vitrification of human sperm in large or small volumes facilitated storage and use of human sperm for long time. However, this procedure was not successful in farm animals. The main finding in the studies is higher sensitivity of these kinds of sperm to sucrose osmolarities. Preliminary studies showed a reversible effect of sucrose on goat epididymal sp...

Vol. 11 | No. 02 | MarchApril 2014 | UROLOGY JOURNAL Vitrification‎has‎brought‎about‎important‎changes‎in‎cryopreservation‎and‎human‎fertility‎preservation.‎Easiness‎and‎speed‎and‎no‎need‎for‎costly‎freezing‎technologies‎ are‎reasons‎for‎its‎rapid‎development.‎Vitrification‎is‎the‎solidification‎of‎a‎liquid‎ without‎crystallization.‎As‎cooling‎continues,‎however,‎the‎molecular‎waves‎in‎the‎liqu...

Over the past 30 years many cryopreservation procedures have been applied to oocytes, embryos, sperm, ovarian and testicular tissue. Over this time many, often specialized, terms have been developed for all aspects of these procedures. This can make it difficult for readers who are not familiar with the terminology or protocols to compare and evaluate different procedures. This paper describes ...