OBJECTIVE To assess the influence of an isotonic lubricant used during sperm sample collection on [1] ease of collection and [2] resultant sperm quality. DESIGN Paired randomized cross-over design. SETTING Tertiary hospital. DONOR(S) Healthy men over 18 years old with normal semen analysis as per World Health Organization 2010 guidelines. INTERVENTION(S) Collection of semen sample from ...

The predictive value of sperm chromatin integrity for pregnancy outcome following in-vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) was studied in 24 men attending a university-based assisted reproductive techniques laboratory using the flow cytometric sperm chromatin structure assay (SCSA). The SCSA is a measure of the susceptibility of sperm DNA to low pH-induced denatu...

Biological mixtures comprised of multiple individuals’ tissues or bodily fluids are commonly encountered and gathered during crime scene evidence collection. Sexual assault samples are one such mixture, usually comprised of epithelial cells and sperm. Sexual assault mixtures may contain a large amount of non-sperm cells in comparison to sperm cells. In such instances, it is imperative to separa...

BACKGROUND Development of a reproducible baboon in vitro fertilization (IVF) system require optimized and reproducible sperm parameters. The objective of this study was to document basic spermatology values and investigate the reproducibility of these variables in the same baboons 1 or 3 months later in a larger number of baboons. METHODS In this prospective study, sperm quality (semen volume...

Sperm motility is an important parameter for success in intracytoplasmic sperm injection (ICSI) treatment. The sperm motility is due to its tail. If the sperm is not moving, it could be dead or alive. Normal live births have been obtained after ICSI with immotile sperm [1,2]. Various procedures have been applied to pick viable sperm for ICSI including, hypo-osmotic swelling (HOS) test [3], addi...

Bulls vary greatly in semen quality, both among bulls and among ejaculates within a bull. Therefore, semen is evaluated routinely using those characteristics that are known to affect fertility. These include general appearance of the semen, volume of the ejaculate, concentration of sperm, motility, metabolic activity, percent live/dead and morphological abnormalities. The minimum levels for a s...

A modified chemotaxis chamber was used to evaluate cellulose acetate/nitrate filters for the measurement of dog sperm motility. The distance travelled into the filters was affected by filter pore size, incubation time and sperm concentration. After storage of spermatozoa at 37 degrees C the distance penetrated into the filters reflected the deterioration of the sample, but was not as sensitive ...

Sperm of the following Neotropical Characiformes fish species were tested for cold storage: Brycon lundii, Piaractus mesopotamicus, Leporinus elongatus, Leporinus friderici, Prochilodus lineatus and Prochilodus marggravii. Each sperm sample was split into two aliquots. The first was placed into a plastic bag with air or oxygen and the second, in a plastic tube with air. The samples were maintai...

Background: Techniques of sperm preparation require centrifugation to separate spermatozoa from the seminal plasma. The centrifugation can induce damage to the spermatozoa and increase reactive oxygen species(ROS) productionspecially in leukospermic speciemens that are associated with impaired function and fertilizing capacity. However in ICSI, fertilization can occure with damage DNA sperm but...

Even though a practical in vitro semen assay for determining bull fertility would be of great benefit to reproductive programs [6], it is unlikely that the evaluation of a single sperm characteristic may reflect the real sperm fertilization capacity of a semen sample. Hence, finding a set of sperm characteristics considered important for predicting conception rate would be a useful method of mo...