the present work examines leaching of lipoxygenase-1 from soybean flour employing stirred tank vessel. the effect of operating parameters, impellers speed, operational period, temperature, ph and scale-up were considered.the acetic acid ph: 5.2 increased the leaching of lipoxygenase- 1.the sensitivity of agitator speed and geometrical-scale-up on the enzymeleaching has been conducted. the effec...