In this report we describe a novel approach to the mass spectrometric analysis of whole proteins from gels. The strategy consists of three components: conventional SDS-PAGE gels, reversible negative staining procedures, and passive elution of proteins from gels followed by mass spectrometric analysis. Protein bands are excised from SDS-PAGE gels, destained, and extracted. For gel loadings > or ...

Quantitative Bioanalysis Liquid separations coupled to targeted mass spectrometric measurements of bioactive compounds BJÖRN ARVIDSSON

When a new spectrometric method is introduced in the literature, the method is investigated initially with instruments with a number of different designs. Each new application of the spectrometric method requires a set of trial experiments to optimize the instrumental parameters. Eventually the different applications and instrumental designs assist in establishing the fundamental advantages and...