The design or investigation of fluorescence probes continues to receive attention with respect the diverse applications spectrofluorometry. Depending on highly sensitive character, spectroscopy-based methodologies have been widely used in recent years different sciences, including analytical, environmental, and medicinal chemistry areas. In our previous works, we shown iron (III) selective on-o...

in the egg shells when the eggs were studied in UV-light (Fig. 1). Control eggs did not fluoresce. The concentration of oxytetracycline in the egg shell, albumen, and yolk was measured by UV-absorption spectrofluorometry (Hayes and DuBuy, 1964). Oxytetracycline was deposited mainly in the shell (0.182 ± 0.028 iig./gm., mean ± SD), and albumen (0.190 ± 0.030 !*g./gm.) whereas the yolk contained ...

Automated methods are described for extracting drugs from urine and for subsequently detecting them by ultraviolet or visible absorptiometry, or by spectrofluorometry. Standard Technicon AutoAn#{225}lyzer quipment has been adapted for use in the extraction processes; and manifolds for barbiturates, morphine, and bases are described. For drugs extracted from urine, the limits of detection were 2...

The occurrence of 2 gibberellin-like substances was demonstrated in the developing banana fruit, Musa sapientum, Linn. Chemical and biological evidence led to the tentative identification of the 2 compounds as GA(7) and GA(x) (previously isolated from citrus fruits). Support for such identification was obtained from thin layer chromatography, gradient elution column chromatography, spectrofluor...

The initial rate of Cu2+ movement across the thylakoid membrane of pea (Pisum sativum) chloroplasts was directly measured by stopped-flow spectrofluorometry using membranes loaded with the Cu(2+)-sensitive fluorophore Phen Green SK. Cu2+ transport was rapid, reaching completion within 0.5 s. The initial rate of uptake was dependent upon Cu2+ concentration and saturated at about 0.6 microm total...