Picoa species are hypogeous desert truffles, which can be found in semi-arid ranges of North Africa, West Asian, South of Europe and Middle East, including Iran (Moreno et al. 2000, Jamali & Banihashemi 2012, 2013). Picoa species form symbiosis mainly on roots of annual and perennial herbaceous plants of the Helianthemum, including H. ledifolium and H. salicifolium var. salicifolium (Jamali & B...

serology and dna techniques are employed for human leukocyte antigen (hla) typing in different transplant centers. results may not always correlate well and may need retyping with different technique. all the patients (with aplastic anemia, thalassemia, and immunodeficiency) and their donors, requiring hla typing for bone marrow transplant were enrolled in the study. serological hla typing was ...

We evaluated the usefulness of PCR assays that target the internal transcribed spacer (ITS) region for identifying mycobacteria at the species level. The conservative and species-specific ITS sequences of 33 species of mycobacteria were analyzed in a multialignment analysis. One pair of panmycobacterial primers and seven pairs of mycobacterial species-specific primers were designed. All PCRs we...

Background: Species identification in commercially processed food and feed products is one of the important issues. This study was conducted to develop a genetic method for the detection of pig and cattle species in processed food and feed products using newly designed species-specific primers targeting mitochondrial 12S rRNA gene fragments. Methods: Two sets of specific primers were designed ...

armillaria root and crown rot is an important disease of woody plants including fruit, forest and ornamental trees in different regions. the disease is common in isfahan and causes economical losses on a broad range of fruit trees. there only a few tolerant varieties to disease, and there is no efficient method in controlling the disease. therefore, detection of the pathogen from soil is necess...

Invasive aspergillosis (1 is a life-threatening condition in immunocompromised patients. An early diagnosis is of great importance because early treatment may resolve this potentially fatal infection. Recently, the polymerase chain reaction (PCR) has been used successfully in detecting specific DNA of several pathogen. In this study, nested PCR was used to detect DNA specific for A!.pergiflus s...

  The aim of this study was to detect and differentiate Theileria annulata and T. lestoquardi (hirci) by PCR. Members of the genus Theileria are tick-borne hemoprotozoan parasites those cause fatal and enervating diseases of cattle and sheep in Iran . In order to develop a specific method for detecting and identification of Theileria species, specific primers from the surface protein (SP) seque...