A lysimeter station has been set up by AMRA for the study of the reactive transport of contaminants in soil at the field-scale. It consists of eight lysimeters that can operate individually and simultaneously. Each lysimeter is a steel cylinder (diameter 1.14m, height 2m) filled with an undisturbed soil monolith, whose upper surface is directly exposed to the external environmental agents. A se...

Microbial electrosynthesis (MES) is a potential technology for CO2 recycling, but insufficient information available on the microbial interactions underpinning electrochemically-assisted reactions. In this study, MES reactor was operated 225 days alternately with bicarbonate or as carbon source, under batch continuous feeding regimens, to evaluate response of communities, and their productivity...

A novel oscillating bioreactor, BelloCell, was successfully applied for the cultivation of Vero cells using serum-free medium, and the production of Japanese encephalitis virus. The BelloCell requires no air sparging, pumping, or agitation, and thus provides a low shear environment. Owing to its simple design, BelloCell is extremely easy to handle and operate. Using this BelloCell (500 ml cultu...

Butanol and 1,3-propanediol (1,3-PDO) are simultaneously produced by Clostridium pasteurianum from glycerol. In this study, random chemical mutagenesis of C. pasteurianum DSM 525 was conducted to improve its tolerance to butanol. Selected nutritional and operational parameters were evaluated to identify strategies that favour the production of each metabolite. From those experiments, it was pos...

This paper presents two-dimensional laboratory experiments performed to study how groundwater flow may affect the injected air zone of influence and remedial performance, and how injected air may alter subsurface groundwater flow and contaminant migration during in situ air sparging. Tests were performed by subjecting uniform sand profiles contaminated with dissolved-phase benzene to a hydrauli...

Contaminated groundwater is a widespread problem often requiring innovative technologies to remediate. In this study, physical models of the air-sparging process are developed, and contaminant recoveries are compared with predictions from a mathematical model. Initial tests used a very fine, porous media (a glass bead-packed column) to represent relatively homogeneous soil samples. Subsequent t...

Air sparging is a popular soil remediation technique that enables the removal of contaminants by diffusing air into saturated zones of soil. The removal process is, however, slow. The goal of this work is to study the effect of electromagnetic (EM) waves — with minimal heat generation — on transport mechanisms such as diffusion, in order to improve airflow or contaminant transport and expedite ...