somatic embryogenesis is a new approache to plant mass in vitro propagation. this method influences by elements and their components in medium. in order to investigate the effects of different media and it,s role on somatic embryogenesis of carrot, a cbd experiment with four replications (including 12 treatments) was conducted. to do this, carrot petioles (with 1 cm dimension) in induction phas...

In cork oak (Quercus suber L.), recurrent embryogenesis is produced in vitro through autoembryony without exogenous plant growth regulators (PGRs); secondary embryos appear on the embryo axis but seldom on cotyledons. Focusing mainly on the histological origin of neoformations, we investigated the influence of the embryo axis and exogenous PGRs on the embryogenic potential of somatic embryo cot...

Background: The beneficial effect of supplementing culture medium with melatonin has been reported during in vitro embryo development of species such as mouse, bovine and porcine. However, the effect of melatonin on the mouse somatic cell nuclear transfer remained unknown. Materials and Methods: In this study, we assessed the effects of various concentrations of melatonin (10-6 to 10-12 M) on t...

At the nonpermissive temperature, somatic embryogenesis of the temperature-sensitive (ts) carrot cell mutant ts11 does not proceed beyond the globular stage. This developmental arrest can be lifted by the addition of proteins secreted by wild-type cells to the culture medium. From this mixture of secreted proteins, a 32-kD glycoprotein, designated extracellular protein 3 (EP3), that allows comp...

present study was conducted to consider the effects of different concentrations of 2,4-d and ba on somatic embryogenesis induction, development and maturation of three strawberry cultivars (kurdistan, parose and camarosa). for this purpose, leaf blade, nodal, petiole, stamen and flower bud calli were cultured on murashige and skoog (ms) medium supplemented with 2, 4 dichlorophenoxyacetic acid (...

Somatic embryogenesis requires auxin and establishment of the shoot apical meristem (SAM). WUSCHEL (WUS) is critical for stem cell fate determination in the SAM of higher plants. However, regulation of WUS expression by auxin during somatic embryogenesis is poorly understood. Here, we show that expression of several regulatory genes important in zygotic embryogenesis were up-regulated during so...

Somatic embryogenesis is a developmental process during which plant somatic cells, under in vitro conditions, produce embryogenic cells that develop into embryos. an efficient method clonal propagation of plants. Mature embryos were used as explant source for callus formation and the produced compact, healthy most mature induced callus. showed similar morphologically to their non-somatic counte...

sugar type and growth regulators are essential fundamental components of tissue culture media. in this study, different concentrations of some carbohydrate and growth regulators during anther culture and producing somatic embryo of datura stramonium l. was investigated in ms medium. the ms medium supplemented with 0.5 mgl-1 kinetin and 2 mgl-1 2,4-d, and also the medium supplemented with 0.25 m...

Since first described in the mid-eighties somatic embryogenesis of spruce has been extensively utilized both as a tool for mass propagation and as a model system for the investigation of structural, physiological and molecular events occurring during embryo development in conifers. Despite the increased efforts to optimize media composition and culture environment, production of somatic embryos...

Macaw palm (Acrocomia aculeata (Jacq.) Lodd. ex Mart.) is a tropical species with multiple uses, including oil supply for biodiesel production. However, structural and physiological studies of the seed are still scarce, as well as in vitro propagation techniques. The aim of this study was to characterize the anatomy, histochemistry and ultrastructure of the seed and the in vitro somatic embryo ...