Technologies to enable Aqueous Solid-Phase Peptide Synthesis (ASPPS).

The chemical approaches to the synthesis of peptides and proteins by the azide coupling 2 have turned out to be one of the safest methods of avoiding racemization. The azide procedure has been widely used for the coupling of difficult amino acid residues such as His, Thr, Ser, Trp, etc., and also particularly for the condensation of peptide fragments using both the maximum and minimum protectio...

1 Chemistry 2 Liquid-phase synthesis 3 Solid-phase synthesis 3.1 Boc SPPS 3.2 Fmoc SPPS 3.3 BOP SPPS 4 Solid supports 4.1 Polystyrene resin 4.2 Polyamide resin 5 Protecting groups 5.1 Fmoc protective group 5.2 Boc protective group 5.3 Benzyloxy-carbonyl (Z) group 5.4 Alloc protecting group 5.5 Lithographic protecting groups 6 Activating groups 6.1 Carbodiimides 6.2 Aromatic oximes 7 Synthesizin...

  Introduction: Bombesin (BN), a 14-amino acid neuropeptide, shows high affinity for the human GRP (gastrin releasing peptide) receptors, which are overexpressed by a variety of cancers, including prostate, breast, pancreas, gastrointestinal, and small cell lung cancer. Aim was to prepare [6-hydrazinopyridine-3-carboxylic acid (HYNIC0), D-Tyr6, D...

Microcin J25 (MccJ25) is a small ribosomally synthesized antimicrobial peptide that is produced by Enterobacteriacea family especially E.coli. The present study focuses on preparation and evaluation of in vitro antimicrobial and biological properties of a new peptide derived from MccJ25. We prepared a MccJ25-derived peptide containing 14 amino acids and a single intra-molecular disulfide bond a...

Microcin J25 (MccJ25) is a small ribosomally synthesized antimicrobial peptide that is produced by Enterobacteriacea family especially E.coli. The present study focuses on preparation and evaluation of in vitro antimicrobial and biological properties of a new peptide derived from MccJ25. We prepared a MccJ25-derived peptide containing 14 amino acids and a single intra-molecular disulfide bond a...

background: ubi 29-41 (a derivative of antimicrobial peptide ubiquicidin) labelled with 99mtc is reported to discriminate between bacterial infections and sterile inflammatory processes. in this study, three lyophilized kit were performed, one of them based on the direct labelling with only sncl2 as reducing agent , and other two based on 6- hydrazinopyridine-3-carboxylic acid (hynic) and trici...

An in-house developed automated synthesis suite was used to prepare a library of 72 tetrapeptide derivatives, the starting materials for pharmaceutically attractive pentapeptides, employing a convergent strategy. An initial set of 18 dipeptides were synthesized on a large-scale (100-1000 g) using automated synthesis workstations, and then 72 tetrapeptides were synthesized on a medium scale (5-1...