Background: Withania somnifera L. Dunal, (Ashwagandha) belongs to the family Solanaceae and is known as ‘Indian ginseng’ in the traditional system of medicine. Objectives: In the present investigation, comparative analysis was carried out between two different ecotypes of W. somnifera related to total soluble protein and antioxidant enzyme activity [catalase (CAT), s...

A separable and highly-stable enzyme system was developed by adsorption of phenylalanine ammonia lyase (PAL) from Rhodotorula glutinis in amino-functionalized macroporous silica gel and subsequent enzyme crosslinking. This resulted in the formation of cross-linked enzyme aggregates (PAL-CLEAs) into macroporous silica gel (MSG-CLEAs). The effect of adsorptive conditions, type of aggregating agen...

withania somnifera (l.) dunal is an erect evergreen shrub commonly known as ashwagandha. it is widely used in ayurvedic, and in the traditional pharmacopeia system of india. it is one of the major ingredients in many formulations prescribed for a variety of musculo-skeletal conditions including arthritis and rheumatism. in the present study, variations in the quality and quantity of proteins an...

Pharmacological evidence implicates trans-cinnamic acid as a feedback modulator of the expression and enzymatic activity of the first enzyme in the phenylpropanoid pathway, L-phenylalanine ammonia-lyase (PAL). To test this hypothesis independently of methods that utilize potentially non-specific inhibitors, we generated transgenic tobacco lines with altered activity levels of the second enzyme ...

the human erythrocyte is a rich raw material for the purification of cu-zn superoxide dismutase (sod). we applied a simple and rapid procedure for the purification of sod from human erythrocytes by ion exchange chromatography. the purified sod had a specific activity of 2285.6 u/mg protein and gave a single band on polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (sd...

Abstract Growth of bionanotechnology requires functional materials, which can lower the costs and be modified to specific reaction. Conjugations nanoparticles enzymes form efficient products multi-material enzyme-nanocomplexes, controlled from synthesis application provide predicted results. Multimetallic nanooxides ZnO–CuO, ZnO–MnO, ZnO–MnO–CuO were obtained by precipitation method with sonica...

Salinity is an important environmental factor that limits plant growth and production. Grape is classified as salt sensitive plants. The object of this study was to evaluate effects of salinity on membrane lipid peroxidation, antioxidant components, and antioxidative enzymes activity in ten grape genotypes native to the regions around Urmia Salt Lake.Malondialdehyde content and protective enzym...

Pot marigold (Calendula officinalis L.) is widely used due to its various biological activities to treat diseases as an analgesic, anti-diabetic, anti-ulcer and anti-inflammatory agent. To ameliorate the adverse effects of water stress on this medicinal plant, foliar application of glycine betaine (GB) and L-arginine (LA) was ...

Background: The manipulation of mineral nutrition may affect secondary metabolite production and thus determine the nutritional value food/medicinal plants. Objectives: present study investigated impact nitrogen supply on metabolites physiological parameters Hypericum androsaemum plants grown in sand culture. Methods: Four-week were irrigated by full-strength Hoagland solution (control), nitrat...