We describe a rapid, simple, and reliable procedure for routine isolation of Mycobacterium leprae DNA from slit-skin swab specimens. This one-step DNA extraction method is based on the utilization of ChelexR 100, a chelating ion exchange resin. Slit-skin swab specimens from seven untreated leprosy patients at Baba Baghi Leprosy Hospital in Iran were processed by this procedure. The polymer...

For cytogenetics of pig (Sus scrofa domestica) and the influence of chromosome aberrations on pig production, high interest exists in flow sorted chromosomes for gene mapping, to establish DNA-libraries, or to produce DNA-probes. Flow karyotyping and sorting as well as slit scan flow analysis of metaphase chromosomes of an abnormal cell type carrying a translocation marker chromosome 6/15 are d...

Podocytes are specialized epithelial cells covering the basement membrane of the glomerulus in the kidney. The molecular mechanisms underlying the role of podocytes in glomerular filtration are still largely unknown. We generated podocin-deficient (Nphs2-/-) mice to investigate the function of podocin, a protein expressed at the insertion of the slit diaphragm in podocytes and defective in a su...

sublingual allergen immunotherapy (slit) is considered to be safer and more convenient than subcutaneus immunotherapy. slit trials with house dust mites involving patients with allergic rhinitis (ar) and asthma reported discordant results. the aim of the study was to investigate the clinical efficacy and safety of slit with dermatophagoides pteronyssinus (d.pt) extract produced in serbia and pa...

The present work numerically investigates vortex-induced vibrations (VIV) of a two-dimensional circular cylinder with an axisymmetric slit at Reynolds number 500. study examines the effects shape (i.e., converging, diverging, and parallel slits), effect slit-area ratios, slit-width, its angle freestream velocity on aerodynamic forces, vibration response, associated flow characteristics. results...

Slit cleavage into N-terminal and C-terminal polypeptides is essential for restricting the range of Slit activity. Although the Slit cleavage site has been characterized previously and is evolutionally conserved, the identity of the protease that cleaves Slit remains elusive. Our previous analysis indicated that Slit cleavage is essential to immobilize the active Slit-N at the tendon cell surfa...

Eroded sediments from catchment areas transport by streams and deposit in downstream less steep areas. Despite reducing the useful life of various hydraulic structures including reservoir dams, flood walls, and bridges, this phenomenon changes the main river hydraulic conditions and increases the flood prone farms in the riverbank area. Check dams are among the structures that can prevent form ...