A single locus system of 6 microsatellite markers was evaluated for paternity testing. A nonradioactive method based on peroxidase labeling of a DNA probe was used to estimate the allele frequency of markers D1S216, D3S1217, D7S480, D9S157, D13S153, and D16S422 by genotyping 1134-1698 chromosomes. The number of detected alleles were 22, 15, 23, 10, 16, and 19, respectively, and the allele frequ...

Abstract. Hidayat A, Rahayu ES, Abdullah M, Retnoningsih A. 2021. Microsatellites to reveal genetic diversity and distinguish four mangoes of Tegal District, Central Java, Indonesia. Biodiversitas 22: 3467-3473. The Wirasangka mango is District’s flora identity, which requires recognition, its existence deserves be preserved. However, based on the morphology fruit, most people still have diffic...

The identification of molecular markers linked to leaf curl virus (CLCuV) disease resistance in cotton has the potential to improve both the efficiency and the efficacy of selection in cotton breeding programs. Genetic analysis suggested that CLCuV resistance is controlled by a single dominant gene. In this study, an interspecific F2 population derived from a cross of Gossypium barbadense and G...

the population genetic structure of the persian sturgeon (acipenser persicus) in the sefidrud and gorganrud river watersheds was analyzed based on the characterization of microsatellite markers. one hundred fin clip samples of persian sturgeon from the two regions were collected. four microsatellite loci (ls68, spl168, spl173, and afu68) were analyzed for molecular characterization, resulting i...

The mandarin fish is a popular fresh water food fish in China. Fifty-three polymorphic microsatellite markers were isolated through construction of an enriched library of genomic DNA of Siniperca chuatsi (Percichthyidae). We found 2 to 7 alleles per locus. The observed and expected heterozygosity values varied from 0.059 to 1.000 and from 0.305 to 0.818, respectively. The polymorphic informatio...

  The objective of this study was to analyse the population genetic structure of the Persian sturgeon (Acipenser persicus) in Sefidrud and Gorganrud rivers watershed based on the characterization of microsatellite markers during 2006 – 2008. 100 samples of Persian sturgeon were collected from two regions. Four microsatellite loci ( Ls 68 , Spl 168 , Spl 173 and Afu 68 ) were analyzed for the mo...

  The objective of this study was to analyse the population genetic structure of the Persian sturgeon (Acipenser persicus) in Sefidrud and Gorganrud rivers watershed based on the characterization of microsatellite markers during 2006 – 2008. 100 samples of Persian sturgeon were collected from two regions. Four microsatellite loci ( Ls 68 , Spl 168 , Spl 173 and Afu 68 ) were analyzed for the mo...

this study aimed to investigate the contribution of four common dfnb (“dfn” for deafness and “b” for autosomal resessive locus) loci and gjb2 gene mutations (exon 2) in hearing impairment in individuals living in markazi and qom provinces of iran. forty consanguineous iranian families with at least three affected individuals in family or pedigree who suffer from an autosomal recessive non-syndr...

We used recently developed microsatellite DNA markers to explore the population genetic structure of the malaria vector, Anopheles moucheti. Polymorphism at 10 loci was examined to assess level of genetic differentiation between four A. moucheti populations from South Cameroon situated 65-400 km apart. All microsatellite loci were highly polymorphic with a number of distinct alleles per locus r...

Nineteen di- and tetranucleotide and one trinucleotide microsatellite DNA markers were isolated from the Galápagos mockingbird (Mimus parvulus) and tested for cross-species amplification in the other three mockingbird species in the Galápagos. In addition, primers for two microsatellite loci previously developed for Mimus polyglottos were redesigned to obtain shorter amplification fragments. Th...