Camptothecin (CN) is active against several experimental tumors and has also been studied clinically. We report here the effect of CN on LI210 cells and asynchronous and synchronous DON cells in culture. CN was toxic both to LI210 cells (0.06 ¿ig/ml,2-hr exposure) and DON cells (0.15 Mg/ml, 1-hr exposure), and it inhibited DNA and RNA synthesis more than it inhibited protein synthesis. CN was ...

Dissolved organic nitrogen (DON) is present in soils and streams around the world. We are only now beginning to explore the important roles this chemically heterogeneous mixture of compounds plays in terrestrial nitrogen (N) cycling. Over centuries, DON leaching may represent a significant "leak" of N, which occurs because plants and microbes cannot prevent DON losses, even in times of high N d...

The trichothecene mycotoxin deoxynivalenol (DON) is a common contaminant of wheat, barley, and maize. New strategies are needed to reduce or eliminate DON in feed and food products. Microorganisms from plant and soil samples collected in Blacksburg, VA, USA, were screened by incubation in a mineral salt media containing 100 μg/mL DON and analysis by gas chromatography mass spectrometry (GC/MS)....

Trichothecene mycotoxins such as deoxynivalenol (DON) are known to modulate the immune function, but to this date only few studies have been conducted with farm animals, especially pigs, which are regarded as most susceptible to this mycotoxin. Mitogen-stimulated proliferation of porcine blood lympho­ cytes was measured using two different assays, the MTT cleav­ age (metabolic activity) and the...

Mycotoxins are secondary metabolites of fungi that grow on a variety of grains, feedstuffs and animal feed. Feed containing moderate to high levels of mycotoxins cause clinical symptoms in most animals, ranging from organ damage to acute mortality. Consumption of low levels of contaminated feed may result in subclinical problems such as immunosuppression. More than 500 mycotoxins have been iden...

WE STUDIED THREE DIFFERENT TRICEPIROS: (Don Santiago x Don Noé), (Cumé x Horovitz) and (Cumé x Don Noé). The tricepiro (Don Santiago x Don Noé) was obtained by crossing the triticale Don Santiago INTA (AABBRR, 2n = 6x = 42) with the trigopiro Don Noé INTA (AABBDDJJ, 2n = 8x = 56). The number of chromosomes for the F(1) was 2n = 49, the most frequent meiotic configuration being 14 bivalents and ...

Two experiments were conducted to characterize the progression of deoxynivalenol (DON)-induced growth suppression and to investigate algae-modified montmorillonite clay (AMMC) as a means to alleviate the effects of DON in nursery pigs. In both experiments, naturally DON-contaminated wheat was used to produce diets with desired DON levels. In Exp. 1, 280 barrows and gilts (10.0 ± 0.2 kg BW) were...