Analysis of the extent and distribution of genetic diversity in crop plants is essential for optimizing sampling and breeding strategies. Several methods were identified for the existence of diversity in maize for the aim of perfect selection based on great diversity among maize genotypes. In this study, Inter-simple sequence repeat (ISSR) markers were used to assess genetic diversity in a sele...

The pathogenicity of 47 isolates of Sclerotinia sclerotiorum from oilseed rape (Brassica napus L.) in Anhui, China, was tested by detached leaf inoculation using the susceptible rape cultivar Wanyou-14. All isolates were pathogenic to the cultivar and could be grouped into 3 categories based on the lesion length on the leaves tested: weak pathogenicity type, intermediate pathogenicity type, and...

Based on the purpose of conservation planning for native species, sixteen populations of cattle, goat andsheep were analyzed by amplification of genomic DNA using inter-simple sequence repeat (ISSR) markers toestimate of genetic structure. DNA samples of 275 animals were collected to Paccess their genetic content.The polymorphism information content (PIC) values and genetic di...

We have identified a fast and easy method for finding microsatellite markers that utilizes genome screening with inter-simple sequence repeat (ISSR) primers to detect microsatellite regions and to obtain sequence information flanking one side of the microsatellites and a restriction-ligation technique with a specific adaptor to allow sequence walking to obtain sequence information flanking the ...

Mungbean improvement via hybridization requires the identification of true F(1) hybrids from controlled crosses before further generations of selfing/crossing and selection. We utilized inter-simple sequence repeat (ISSR) markers for identifying putative F(1) hybrids from six cross combinations whose morphological characteristics were very similar to those of their respective female paren...

macrophomina phaseolina is an important soil-borne pathogen causing charcoal rot in many important crop plants including sesame, in iran. a total of 60 isolates of m. phaseolina were collected from the main sesame producing regions in ten provinces of iran. the genetic diversity among m. phaseolina populations was estimated using inter-simple sequence repeat (issr), focusing particularly on geo...

Phyllanthus has been widely used in traditional medicine as an antipyretic, a diuretic, and to treat liver diseases and viral infections. Correct genotype identification of medicinal plant material remains important for the botanical drug industry. Limitations of chemical and morphological approaches for authentication have generated the need for newer methods in quality control of botanicals. ...

micropropagated plantlets derived from three different grape rootstock genotypes namely, dogridge (vitis champini), so4 (v. berlandieri×v. rupestris) and ari-h-144 (v. vinifera×v. labrusca) were subjected to randomly amplified polymorphic dna (rapd) and inter simple sequence repeats (issr) analyses in order to evaluate their genetic stability and/or detect likely existing variations among in vi...

Out of fifty-two Iranian nonaflatoxigenic strains of Aspergillus flavus,collected from various substrates (soil and kernel) and sources (peanut, corn and pistachio), fifteen representatives were selected according to their different geographical origins (six provinces: Guilan and Golestan, Ardebil, Fars, Kerman and Semnan) and vegetative compatibility groups (VCGs, IR1 to IR15) for microsatelli...