Shoot meristems used for the study were exercised from the in vitro regenerated shoots cultured on MS medium supplemented with 0.5 mg/L of BAP for multiplication. The sensitivity of the in vitro regenerated was studied using shoot meristems of 0.5 cm. Shoot meristems were cultured on medium containing 10 100 mg/l kanamycin to determine the concentration that was lethal for multiple shoot induct...

Glutathione (GSH) and ascorbate (ASC) are important antioxidants that are involved in stress defence and cell proliferation of meristematic root cells. In principle, synthesis of ASC and GSH in the roots as well as ASC and GSH transport from the shoot to the roots by phloem mass flow is possible. However, it is not yet known whether the ASC and/or the GSH level in roots depends on the supply fr...

Glutathione (GSH) and ascorbate (ASC) are important antioxidants that are involved in stress defence and cell proliferation of meristematic root cells. In principle, synthesis of ASC and GSH in the roots as well as ASC and GSH transport from the shoot to the roots by phloem mass flow is possible. However, it is not yet known whether the ASC and/or the GSH level in roots depends on the supply fr...

More than 50% out of 129 Agave species are endemic to Mexico. Among them, peacockii is among the list threatened that require special protection. In this work, we aimed at developing new supplementary strategies achieve micropropagation and perform cryopreservation in vitro grown shoot-tips A. peacockii. For multiplication, addition two cytokinins, 6-benzylaminopurine (26.6 μM) kinetin (27.84 M...

A protocol for micrografting shoot tips harvested from in vitro shoot cultures directly to transplanted rootstock plants in the greenhouse was developed. Shoot tips of the apple (Malus domestica) cultivars Golden Delicious, Granny Smith and Fuji clone, Nagafu12, were harvested, stored in a water bath then prepared for grafting by cutting the stem immediately below the tip into a wedge shape lea...