نتایج جستجو برای: shoot apex explants were prepared from 4
تعداد نتایج: 7600187 فیلتر نتایج به سال:
Shoot organogenesis and plant establishment has been achieved for Balanites aegyptiaca (L.) Del. from excised root explants. Young root explants obtained from 4 weeks old aseptic seedlings were used for the induction of direct shoot regeneration by incorporating various cytokinins either singly or in combination with auxins in Murashige and Skoog (MS) basal medium. Root explants cultured on MS ...
The present study demonstrates an efficient protocol for in vitro mass propagation of A. curassavica through direct as well as indirect regeneration. The effect of MS and L2 media supplemented with various concentrations and combinations of growth regulators has been studied. The growth regulators used include BAP and kinetin (cytokinins) and IAA, IBA, NAA and 2, 4-D (auxins). MS media proved b...
explants of leaves, petals, anthers, styles and ovaries of c. speciosus, c. cancellatus, c. gilanicus and c. almehensis were cultured on ls media with three different combinations of naa and bap in the dark. callus and neoformant organs (leaf and corm-like structure and ordinary and contractileroots) were fomd on some of these explants. corm explants from these species were also cultured on ms ...
Presence of pharmaceutically important alkaloids in various parts of the plant body made Rauvolfia serpentina an overexploited one and led to its inclusion in Red Data Book of India as an endangered species. Conventional propagation is beset with problems like poor seed viability, low seed germination rate and delayed rooting of cuttings. In vitro clonal propagation may therefore be beneficial ...
One challenge for plant biology has been to identify floral stimuli at the shoot apex. Using sensitive and specific gas chromatography-mass spectrometry techniques, we have followed changes in gibberellins (GAs) at the shoot apex during long day (LD)-regulated induction of flowering in the grass Lolium temulentum. Two separate roles of GAs in flowering are indicated. First, within 8 h of an ind...
This experiment was performed to optimize the medium for tissue culture of Taraxacum kok-saghyz Rodin. Different tissue culture approaches such as shoot regeneration from seed, callus formation from leaf explants and plant regeneration from callus were investigated in this study. All the explants were cultured on MS basal medium supplemented with 20g/l sucrose, 7g/l agar and different plant gro...
the purpose of this study was to compare rooting methods in tissue culture regenerated-clones in sugar beet and their adaptation to environmental conditions and determine clones ploidy stability. two kinds of explants including shoot apex of seedlings and inflorescence stem pieces were used for preparation and propagation of the clones in media. in the next step, three rooting and adaptation me...
Molecular genetic techniques hold great promise to battle various plant biotic and abiotic stresses. Sugar beet (Beta vulgaris L.) is an important recalcitrant crop plants in terms of applying genetic engineering techniques, especially transformation. In an attempt to investigate and optimize sugar beet tissue culture for plant transformation, a 3´2´2 factorial experiment in a completely random...
Background: Chrysanthemum; also commonly known as mums or chrysanths, is one of the most important ornamental crops worldwide. Introducing desirable traits into this valuable plant by the conventional breeding has so far been faced with some restrictions due to the limited gene pool and cross-incompatibility. Therefore, breeders have decided to exploit Agrobacterium-mediated tr...
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