background and objective: over 165 million cases of shigellosis occur in the world each year, mostly in developing countries. outbreaks of shigellosis are associated with poor sanitation, natural calamities, contaminated food and crowded living conditions. in late summer 2006, during the final stage of an outbreak of shigellosis at a vast region of isfahan province, naein & ardestan, our labora...

bacillary dysentery, or shigellosis, is a disease of humans in which the colonic epithelium is invaded by bacteria and subjected to inflammatory destruction. the aim of this study was to develop a polymerase chain reaction(pcr) test for detection of virulent shigella spp.. for this purpose, the primers were designed to amplify a 526-bp internal region of the shigella spp. icsa gene, which encod...

The enteric pathogen Shigella utilizes host-encoded proteins to invade the gastrointestinal tract. Efficient invasion of host cells requires the stimulation of Rho-family GTPases and cytoskeletal alterations by Shigella-encoded IpaC. Following invasion and lysis of the phagosome, Shigella exploits the host's actin-based polymerization machinery to assemble an actin tail that serves as the propu...

The differentiation between Shigella subspecies, and the phylogenetic position of Shigella clones within Escherichia coli clones was determined by analysis of restriction fragment length polymorphisms of rDNA (ribotyping). Seventy-five Shigella strains belonging to the four subspecies and 13 enteroinvasive E. coli (EIEC) strains were compared with the 72 E. coli strains of the ECOR collection, ...

materials and methods s. sonnei and s .flexneri was identified by common microbiological and serological methods. antibiogram with 18 antibiotics were tested for 34 positive cultures by disc diffusion method. the samples showed considerable resistance to antibiotics. antimicrobial effects of cfcs were tested against s. sonnei and s. flexneri by agar-well assay and broth micro dilution methods. ...

background shigellosis as a global human health problem is more severe than other forms of gastroenteritis and causes over a million deaths in developing countries worldwide annually. fatality due to shigellosis is usually due to dehydration and two-third of fatalities are seen among children. the aim of current study was to describe fatal cases of shigellosis due to infection with shigella son...

Shiga toxins (Stx) are commonly produced by Shigella dysenteriae serotype 1 and Stx-producing Escherichia coli. However, the toxin genes have been detected in additional Shigella species. We recently reported the emergence of Stx-producing Shigella in travelers in the United States and France who had recently visited Hispaniola (Haiti and the Dominican Republic). In this study, we confirm this ...

BACKGROUND The entire gastrointestinal tract is protected by a mucous layer, which contains complex glycoproteins called mucins. MUC2 is one such mucin that protects the colonic mucosa from invading microbes. The initial interaction between microbes and mucins is an important step for microbial pathogenesis. Hence, it was of interest to investigate the relationship between host (mucin) and path...

Shigella spp. are exquisitely fastidious gram-negative organisms which frequently escape detection by traditional culture methods. To get a more complete understanding of the disease burden caused by Shigella in Nha Trang, Vietnam, real-time PCR was used to detect Shigella DNA. Randomly selected rectal swab specimens from 60 Shigella culture-positive patients and 500 Shigella culture-negative p...

Bacillary dysentery or shigellosis is mediated by the pathogenic enterobacteria Shigella. Shigella are transmitted through the fecal-oral route, no animal reservoir has been described, and remain a major cause of moderate-severe diarrhea worldwide since no Shigella vaccine is yet commercialized. The design of a pan-Shigella vaccine has been made difficult by the large variety of Shigella specie...