objective: herpes simplex virus (hsvs) is a widespread human infectious agent, responsible for persistent and latent infections. herpes simplex virus infections are usually continually recurrent in the normal population and represent a significant cause of complications in immunocompromised patients. materials and methods: in this study hsvs were propagated in bk cells and more than 502 sample...

Modification of the pH of kaolin-serum treatment produces hemagglutination-inhibition test results comparable with the plaque neutralization test in duck embryo tissue culture; the standard treatment does not.

Background and Aims: Herpes simplex virus (HSV) is responsible for several significant human viral diseases, with severity ranging from subclinical to fatal infection. Herpes simplex virus type 2 (HSV2) infections are more commonly seen in association with the genitalia and surrounding areas, and can be transmitted to newborns during childbirth. Generalized infections in newborns are also predo...

A method for the screening of tetanus and diphtheria antibodies in serum using anatoxin (inactivated toxin) instead of toxin was developed as an alternative to the in vivo toxin neutralization assay based on the toxin-binding inhibition test (TOBI test). In this study, the serum titers (values between 1.0 and 19.5 IU) measured by a modified TOBI test (Modi-TOBI test) and toxin neutralization as...

Background and Aims: Foot-and-Mouth Disease (FMD) is a highly contagious infectious disease of livestock which has made a barrier to hygiene causing severe loss in livestock and their products. The aim of this study was the assessment of antibody response against foot and mouth disease virus  types A13, A15 , O2010, after injection of FMD vaccine candidate produced by Razi Institue. Mater...

To ascertain the prevalence of BHV-1 antibodies among cattle population, Virus neutralization test (VNT) and indirect Enzyme linked immunosorbent assay (ELISA) were employed for the detection of BHV-1 antibodies. Evaluation of these two serological tests was made to find out the rapid, economic and sensitive diagnostic test for detection of BHV-1 infection. A total of 255 serum samples were col...

We have previously described an assay to quantify the serum neutralization of bacterial lipopolysaccharide which is based on a spectrophotometric Limulus amoebocyte lysate test (T.J. Novitsky, P.F. Roslansky, G.R. Siber, and H.S. Warren, J. Clin. Microbiol. 21:211-216, 1985). Studies since have shown that serum samples drawn from patients with leukemia and fever, gram-negative or gram-positive ...

A radioimmunoassay procedure was developed for determining smallpox and vaccinia antibodies in human sera. The test detected and measured both primary and secondary immune responses in persons infected with variola virus or vaccinia virus. The antibody titers obtained by complement fixation, hemagglutination inhibition, plaque reduction neutralization, and radioimmunoassay methods were compared...

In New Zealand, an arbovirus surveillance program has been operating for more than 20 years, which includes testing of cattle with the Akabane virus neutralization test. With the aim to replace this laborious test by an easier-to-perform enzyme-linked immunosorbent assay (ELISA), 2 commercial ELISA kits, ELISA-1 from France (originally from Australia) and ELISA-2 from Japan, were compared, usin...

Objective(s) Herpes simplex viruses (HSVs) have widespread and ubiquitous prevalence in the human population and they have received a great deal of attention due to the range of diseases, they caused as a result of an infection. It seems that the fast and reliable diagnostic methods are needed for detecting the herpes simplex virus type 1 (HSV1) antibodies especially in patients with HSV encep...