We developed a kinetic procedure for determination of amylase activity in serum by use of nephelometric measurements. Light scattering from the substrate, a stable suspension of starch, is decreased as amylase hydrolyzes the starch to soluble fragments. Values obtained for serum amylase correlate closely with those determined by a method in which a starch-dye complex is used. Precision of the n...

Background: Diagnosis of acute pancreatitis is based on raised serum lipase and amylase in the blood. However, levels urinary can be sought for being less invasive. The study aimed to find out diagnostic accuracy compared their association with degree severity pancreatitis. Methods: A randomized clinical control was conducted n=180 patients (18-50 year) Ziauddin PNS Shifa Hospital, Karachi from...

1 “Grigore T. Popa” University of Medicine and Pharmacy, School of Pharmacy, Iaşi, Romania 2 “Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, School of Veterinary Medicine, Iaşi, Romania ABSTRACT. The purpose of the study was to assess the effect of selenium supplementation on serum amylase, lactate dehydrogenase (LDH) and alkaline phosphatase (ALP) activiti...

BACKGROUND Low serum amylase is likely to be associated with obesity and metabolic abnormalities, which are often accompanied by impaired insulin action. However, it is unclear whether low serum amylase is associated with impaired insulin action in clinical settings. Therefore, we investigated the associations of low serum amylase with plasma insulin levels, and obesity-related parameters, incl...

For patients with symptoms of pancreatitis, measurement of amylase in serum reportedly is more sensitive than that of lipase in acute pancreatitis, whereas lipase reportedly is more specific. However, serum lipase activities exceeding the upper reference limit (URL) have been reported for many patients who did not have pancreatitis. I reviewed the serum lipase and amylase concentrations of 493 ...

Muffin et al. (1), in this issue, describe a more specific method for pancreatic ainylase in serum. Amylase of salivary origin is precipitated with a monoclonal antibody, and the amylase remaining is presumed to come from the pancreas. It is likely that their test will be more specific for pancreatic disease. Since the recognition of multiple sources of antylase and of the frequency of hyperamy...

Human salivary amylase and pancreatic amylase were purified and characterized. These amylases gave two bands and one band, respectively, each staining for both protein and sugar, after electrophoresis on sodium dodecyl sulfate--polyacrylamide gel. The relative molecular mass (Mr) or pancreatic amylase was calculated to be 60 000; for the two components (A and B) of salivary amylase the Mr were ...

Levels of red cell, serum acid, and alkaline phosphatases, serum amylase, alanine and aspartate transferase and bilirubin were examined in streptozotocin-induced diabetic rats treated with garlic oil and compared with the corresponding levels in diabetic control rats, normal rats and normal rats on garlic oil. Values of tissue amylase and total protein were also assessed from the pancreas, live...

α-Amylase (1, 4-ƒ¿-D-Glucan glucanohydrolase E.C. 3. 2. 1. 1.) distributes widely in micro organisms, animals and plants. In animals, the characterization of the enzyme was reported using the rat1), mouse2), hamster3), dog4), rabbit5), cat6), kangaroo7), silkworm8) and human9-10). Our previous reports indicated that the serum amylase was released from the parotid gland by the administration of ...

Serum lipase is determined by following turbidity changes during two 1-mm intervals after adding serum to an olive oil emulsion containing desoxy. cholate. The olive oil emulsion is simply prepared and is stable for a month under refrigeration. Our observations confirm the findings of other investigators that increases in serum lipase activity are more accentuated than increases in serum amylas...