نتایج جستجو برای: sensing ion channel 1a

تعداد نتایج: 550163  

Journal: :FEBS letters 2010
Masatoshi Yokokawa Stewart M Carnally Robert M Henderson Kunio Takeyasu J Michael Edwardson

The acid-sensing ion channel (ASIC) 1a is known to assemble as a homotrimer. Here, we used atomic force microscopy to image ASIC1a, integrated into lipid bilayers, at pH 7.0 and pH 6.0. The triangular appearance of the channel was clearly visible. A height distribution for the channels at pH 7.0 had two peaks, at 2 and 4 nm, likely representing the intracellular and extracellular domains, respe...

Journal: :Chemical communications 2015
Ming Wen Xiaoqi Guo Peibei Sun Liang Xiao Juan Li Ying Xiong Jin Bao Tian Xue Longhua Zhang Changlin Tian

The synthesis of fluorescent unnatural amino-acid Anap was optimized and the Anap was incorporated into four sites in an acid-pocket or a transmembrane region of human acid-sensing ion channel-1a (hASIC1a). Combinational Anap fluorescence spectra and patch-clamp electrophysiology data illustrated site-specific conformational responses upon toxin mambalgin-1 binding. This combinational approach ...

Journal: :The Journal of biological chemistry 2006
Marija Vukicevic Gilles Weder Aurélien Boillat Anne Boesch Stephan Kellenberger

Acid-sensing ion channels (ASICs) are neuronal Na(+) channels that are members of the epithelial Na(+) channel/degenerin family and are transiently activated by extracellular acidification. ASICs in the central nervous system have a modulatory role in synaptic transmission and are involved in cell injury induced by acidosis. We have recently demonstrated that ASIC function is regulated by serin...

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