Experiments were conducted to determine the effect of hypothermic 24 h storage on striped bass sperm cell plasma membrane integrity, free intracellular Ca(2+) ([Ca(2+)](i)), mitochondrial membrane potential (ΔΨ(m)), and reactive oxygen species (ROS) formation (oxidation of hydroethidine to ethidium) as determined by flow cytometry; motion activation and ATP concentration as determined by Lucife...

The examination of quality of the sperm ejaculate is one of the most important steps in artificial fertilization procedure. The main aim of semen storage centres is to characterise the best semen quality for fertilization. Reliable information about sperm motility is also one the most important parameters for in vitro laboratory procedures. There exist very expensive automated methods for semen...

semen collection evaluation and addition of preservatives to increase storage period of sperm are essential for successful artificial insemination. this study was conducted on 4rams (2 ghezel merinos and 2 merinos moghani) to evaluate the effect of two diluents in khalatposhan research station. average age of rams was 3 years. rams were trained to serve the artificial insemination and semen sam...

Semen collection evaluation and addition of preservatives to increase storage period of sperm are essential for successful artificial insemination. This study was conducted on 4rams (2 Ghezel Merinos and 2 Merinos Moghani) to evaluate the effect of two diluents in Khalatposhan research station. Average age of rams was 3 years. Rams were trained to serve the artificial insemination and semen sam...

in this research, effect of bovine serum albumin (bsa) on motility and fertility of indigenous rooster spermatozoa was evaluated using 20 and 48 indigenous roosters and hens, respectively. semen was collected and pooled. in the first experiment, the pooled semen was diluted and divided into four aliquots.the treatments were included the diluted semen without bsa and with 1% bsa; in each group, ...

Rooibos (Aspalathus linearis) is a native shrub from South African fynbos and has become very popular in the last decades for its antioxidant and medicinal attributes. Several studies have shown its beneficial properties in numerous cell lines, but to date, the in vitro effects of rooibos extract on sperm cells are still unknown. In this study, boar semen was supplemented with four concentratio...

In this study the effect of extenders and temperatures on sperm viability and fertilizing capacity of boar sperm during long-term storage was investigated. Acrosomal integrity, membrane integrity, motility and hypo-osmotic resistance were evaluated by fluorescence and light microscopy. An in vitro fertilization test was performed to assess the fertilizing capacity of stored spermatozoa. The fiv...

Environmental influences on reproduction and semen production in the buck, the problem of interaction between seminal plasma and egg yolk or milk constituents in diluent, liquid storage and processing of semen for freezing are discussed. A review is given on the use of frozen-thawed semen for artificial insemination (AI) in spontaneous and induced oestrus and factors influencing the fertility.

The aim of the study was to evaluate the effect of semen storage on ferrous ion induced luminescence of boar spermatozoa and to determine the relationship between parameters of this luminescence and lipid peroxidation as measured by malondialdehyde (MDA) contents. Boar semen samples were diluted in Biosolwens extender and stored for 12 days at 15 degrees C. Luminescence and MDA were measured di...

Considerable scope exists for the use of AI in camel breeding programmes but the technology is not fully developed. The fertility of both fresh and liquid-stored (24 h; 4°C) dromedary camel semen diluted in either Green Buffer (GB) or INRA-96 (INRA) was assessed. Different insemination doses were investigated: 150 and 300 x 10 motile sperm for fresh sperm, and 150, 300 and 600 x 10 motile sperm...