Changes at the molecular level always occur at different stages in diseased cells. The detection of these changes is critical for understanding the molecular mechanisms underlying pathogenesis, as well as accurately diagnosing disease states and monitoring therapeutic modalities. Cell-SELEX is a foundational tool used to select probes able to recognize molecular signatures on the surface of dis...

To the Editor: Revolutionizing the traditional Systematic Evolution of Ligands by EXponential Enrichment (SELEX), high-throughput SELEX (HT-SELEX) has become the method of choice for the identification of aptamers—synthetic, single-stranded (ribo)nucleic molecules that bind to a given molecular target. 1 In contrast to traditional SELEX, in which only data from the last selection cycle is obtai...

UNLABELLED Aptamers are 'synthetic antibodies' that can bind to target molecules with high affinity and specificity. Aptamers are chemically synthesized and their discovery can be performed completely in vitro, rather than relying on in vivo biological processes, making them well-suited for high-throughput discovery. However, a large fraction of the most enriched aptamers in Systematic Evolutio...

In vitro experiments that characterize DNA-protein interactions by artificial selection, such as SELEX,are often performed with the assumption that the experimental conditions are equivalent to natural ones. To test whether SELEX gives natural results, we compared sequence logos composed from naturally occurring leucine-responsive regulatory protein (Lrp) binding sites with those composed from ...

Aptamers are molecules identified from large combinatorial nucleic acid libraries by their high affinity to target molecules. Due to a variety of desired properties, aptamers are attractive alternatives to antibodies in molecular biology and medical applications. Aptamers are identified through an iterative selection-amplification process known as systematic evolution of ligands by exponential ...

SELEX (systematic evolution of ligands by exponential enrichment) is a very powerful method for determining the binding site of a protein on RNA. It relies on the ability of an RNA-binding protein to select high-affinity RNA ligands from a randomized pool of RNAs. SELEX experiments are performed over several "rounds," with each round resulting in increased enrichment of RNAs capable of binding ...

Aptamer is a protein-binding oligonucleotide which can be in vitro selected by systematic evolution of ligands by exponential enrichment (SELEX), and it has the advantages of high affinity, low immunogenicity and easy synthesis as a promising class of antibody surrogates. The microfluidic SELEX performs an improved multiplex platform which can run a number of aptamer selection in parallel and s...

SELEX-Seq is now the optimal high-throughput technique for characterizing DNA-binding specificities of transcription factors. In this study, we introduced an improved EMSA-based SELEX-Seq strategy with several advantages. The improvements of this strategy included: (1) using a FAM-labeled probe to track protein-DNA complex in polyacrylamide gel for rapidly recovering the protein-bound dsDNA wit...