نتایج جستجو برای: selectable marker
تعداد نتایج: 130804 فیلتر نتایج به سال:
abstract background: selectable marker gene (smg) systems are critical for generation of transgenic crops. transgenic crop production background: selectable marker gene (smg) systems are critical for generation of transgenic crops. transgenic crop production without using smg is not economically feasible. however, smgs are non-essential once an intact transgenic plant has been established. elim...
Isolation of recombinant viruses after DNA transfection remains the most time-consuming and labor-intensive feature in the production of vaccinia virus (VV) recombinants. This procedure has been aided by the design and incorporation of both positive and negative selectable markers into the insertion vectors. Use of these selectable markers is not always feasible or desirable. In the absence of ...
BACKGROUND Gene disruption by targeted integration of transfected constructs becomes increasingly popular for studies of gene function. The chicken B cell line DT40 has been widely used as a model for gene knock-outs due to its high targeted integration activity. Disruption of multiple genes and complementation of the phenotypes is, however, restricted by the number of available selectable mark...
Abiotic stresses such as drought, salt, high temperature, chemical toxicity and oxidative stress are considered serious to agriculture and adversely affect the growth and reproduction of crops. Production of transgenic rice was studied utilizing Agrobacterium-mediated strain EHA105 and p6-ubi vector. This vector contains spectinomycin resistance gene as bacterial selectable marker, hygromycin...
Since the maize transposon Ac can relocate to a new location within the genome, it has been used in removing selectable markers in transgenic plants. We previously developed an inducible transposon system to truncate a selectable marker in transgenic plants by locating one end of the transposon in the intron of the marker gene (glyphosate tolerant epsps gene). We have now improved upon this sys...
Genome engineering can be used to produce bacterial strains with a wide range of desired phenotypes. However, the incorporation of gene-sized DNA fragments is often challenging due to the intricacy of the procedure, off-target effects, and low insertion efficiency. Here we report a genome engineering method enabling the continuous incorporation of gene-sized double-stranded DNAs (dsDNAs) into t...
A limitation of transfection of malaria parasites is the availability of only a low number of positive selectable markers for selection of transformed mutants. This is exacerbated for the rodent parasite Plasmodium berghei as selection of mutants is performed in vivo in laboratory rodents. We here report the development and application of a negative selection system based upon transgenic expres...
Genetic studies in Borrelia burgdorferi have been hindered by the lack of a nonborrelial selectable marker. Currently, the only selectable marker is gyrB(r), a mutated form of the chromosomal gyrB gene that encodes the B subunit of DNA gyrase and confers resistance to the antibiotic coumermycin A(1). The utility of the coumermycin-resistant gyrB(r) gene for targeted gene disruption is limited b...
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