BACKGROUND Secretory proteins of IgE receptor activated mast cells and basophils play a pivotal role in the generation of immediate and long term immune responses in allergy and type I hypersensitivity. OBJECTIVE The present study aims to generate a 2-D map and profile of proteins secreted from a high secretory variant of the rat basophilic leukemia cell line, RBL-2H3.1, which in view of the ...

Newly synthesized secretory granule content proteins are delivered via the Golgi complex for storage within mature granules, whereas constitutive secretory proteins are not stored. Most soluble proteins traveling anterograde through the trans-Golgi network are not excluded from entering immature secretory granules, whether or not they have granule-targeting signals. However, the ;sorting-for-en...

The production of a functional protein does not end with the termination of the growing polypeptide chain. This, while clearly true of all proteins, is particularly obvious in the case of secretory proteins. While all proteins must fold to a functional conformation, and many must assemble into some supramolecular structure, secretory proteins must also make their way across membranes and throug...

Abstract In human cells, one third of all polypeptides enter the secretory pathway at ER. This process involves N-terminal signal peptides or internal transmembrane helices in precursors and hundred cytosolic ER proteins, which facilitate their import processing. past fifty years four pathways for targeting to Sec61 channel plus various allosteric effectors supporting stand alone membrane prote...