HIV-1- and cytomegalovirus (CMV)-specific CD4 T-cell-mediated antiviral immunity was evaluated by assessing the frequency of interleukin 2 (IL-2)- and interferon gamma (IFN-gamma)-secreting cells following antigen-specific stimulation in blood and lymph node. HIV-1-infected subjects with progressive disease at early stage of infection with no previous history of antiretroviral therapy (ART), su...

Tumor-associated macrophages (TAMs) are important components in tumor microenvironment. This study intended to explore the influence of TAMs on cervical cancer cells proliferation and migration. The expression levels markers, CD68 CD163, tissues were examined by immunohistochemistry increased with progression lesions (p < 0.05). M2-like phenotype (PMA(Polymethacrylate) induced THP-1 cells) noti...

In a clonal analysis of the IgE response, it was found that a small proportion of primary or nonimmune B cells in spleen and mesenteric lymph nodes can be stimulated by antigen to produce IgE-secreting clones. In addition, there appears to be no substantial difference in the frequency of such cells between the classical low and high IgE responder strains. An analysis of immune, or memory, B cel...

OBJECTIVE The purpose of this study was to detect and enumerate T cells secreting type 1 and 2 cytokines in the peripheral blood of patients with oral lichen planus (OLP) and in healthy controls. SUBJECTS AND METHODS The study group consisted of 80 OLP patients and 80 healthy individuals. Cytokine secreting T cells were detected using ELISPOT assay. RESULTS There was a statistically signifi...

Functional and phenotypic characterization of virus-specific CD8 T cells against cytomegalovirus, Epstein-Barr virus, influenza (flu), and HIV-1 were performed on the basis of the ability of CD8 T cells to secrete IFN-gamma and IL-2, to proliferate, and to express CD45RA and CCR7. Two functional distinct populations of CD8 T cells were identified: (i) dual IFN-gamma/IL-2-secreting cells and (ii...

A new method to quantitate circulating cells secreting immunoglobulin (Ig) of each type of the different classes by hemolytic plaque technique is reported. The ratio of kappa (k)-secreting cells and lambda(lambda)-secreting cells of the different lg classes in normal individuals is almost equal to that of kappa:lambda light chains in corresponding serum lg class, whereas the ratio in plasma cel...

The pituitary gonadotropic adenoma is an developed́ in the cells of anterior pituitary. It can be secreting releasing excess blood and urine; products its secretion are gonadostimulines (F.S.H. L.H.) or non-secreting this case only histo- cytochemical analysis will able to allow identification. We report two cases macroadenoma with LH-FSH immunostaining. Pituitary magnetic resonance imaging reve...

a 7-year-old castrated male german shepherd dog was presented with a 10-day history of intermittent lethargy, weakness and episodic seizures. laboratory findings after admission revealed hypoglycaemia and hyperinsulinaemia. provisional diagnosis of insulinoma was made based on the presence of concurrent hypoglycaemia and elevated insulin level. in exploratory laparotomy a small nodular mass was...

Therapeutic models using gene transfer into tumor cells have pursued three objectives: (1) to induce rejection of the tumor transduced with therapeutic genes, (2) to induce immune-mediated regression of metastatic disease, and (3) to induce long-lasting immunity to protect against challenge with tumor cells or clinical regrowth of micrometastatic disease. Because in vivo therapy for patients wi...

The objective of this study was to determine the effect of neurotrophin-secreting Schwann cell implants on the urinary bladder after spinal cord contusion. One hour after severe spinal cord contusion at the T8 to T11 level, carbon filaments containing nonsecreting Schwann cells, brain-derived neurotrophic factor (BDNF)-secreting Schwann cells, neurotrophin-3 (NT-3)-secreting Schwann cells, or S...