Carbonic anhydrases (CAs) are enzymes that catalyse the reversible hydration of CO2 to bicarbonate. CA XII is considered a potential biomarker of tumor cells and a promising target for specific therapies. The aim of the current study was to develop new monoclonal antibodies (MAbs) against human recombinant CA XII and evaluate their diagnostic potential. An extracellular catalytic domain of huma...

Background and aims: Carbonic anhydrase XII (CA XII) is a membrane-bound isozyme expressed in some normal human tissues, upregulated in some cancers, and is showed to be a hypoxia-inducible gene product. The expression of CA XII mRNA has been demonstrated in mouse kidney. This study concentrated on the research of the expression of CA XII in all mouse tissues, and later on in rat brain tissues....

BACKGROUND Although recent studies described important roles for carbonic anhydrase (CA) XII in epithelial carcinogenesis and tumor behavior, a consensus has not yet been reached regarding its clinicopathological significance in esophageal squamous cell carcinoma (ESCC). In the present study, we investigated its prognostic significance in ESCC. MATERIALS AND METHODS An immunohistochemical ana...

Previous studies have suggested that human platelets can promote the activation of factor XI by two different mechanisms, one requiring factor XII and ADP-treated platelets and the other requiring collagen-treated platelets in the apparent absence of factor XII. To investigate these hypotheses. isolated platelets were tested for their capacity to promote the activation and cleavage of purified ...

OBJECTIVE To evaluate factor XII levels in women with recurrent pregnancy loss (RPL) in a tertiary referral hospital. METHODS Women who were referred to our hospital for two consecutive abortions or three abortions in between 2007 and 2013 were included in this retrospective observational study. Women were further grouped according to factor XII levels, as <60% and ≥ 60%. RESULTS Mean facto...

Mine water is a menace to coal mining. Transient Electromagnetic Method (TEM) is a relatively quick and efficient method to detect the water yield property of a targeted layer. Based on mine-oriented 3D TEM observing system, the signal of induced voltage is obtained. TEM 3D data volume can be calculated through the calculation of late-period resistivity and time-depth conversion. After setting ...

The inspiratory drive to hypoglossal (XII) motoneurons originates in the caudal medullary intermediate reticular (IRt) region. This drive is mainly glutamatergic, but little is known about the neurochemical features of IRt XII premotor neurons. Prompted by the evidence that XII motoneuronal activity is controlled by both muscarinic (M) and nicotinic cholinergic inputs and that the IRt region co...

..................................................................................................................................................II CONTENT...................................................................................................................................................III FIGURES......................................................................................

Nucleus raphé obscurus (NRo) modulates hypoglossal (XII) nerve motor output in the in vitro transverse brain stem slice of neonatal rats (1-5 days old); chemical ablation of NRo and its focal CO(2) acidification modulated the bursting rhythm of XII nerves. We microinjected a 4.5 mM solution of kainic acid into the NRo to disrupt cellular activity and observed that XII nerve activity was tempora...

A monoclonal antibody specific for the chicken alpha 1 (XII) collagen chain was used to immunolocalize type XII collagen in the corneoscleral of 17-19-day-old chicken embryos. These immunofluorescence studies localized type XII collagen around the scleral cartilages and ossicles. There was also a striking positive staining in the scleral spur and stroma of the corneolimbus beneath the external ...