According to the potential anti-hepatoma therapeutic effect of Schisandra chinensis polysaccharides presented in previous studies, a bioactive constituent, homogeneous Schisandra chinensis polysaccharide-0-1 (SCP-0-1), molecular weight (MW) circa 69.980 kDa, was isolated and purified. We assessed the efficacy of SCP-0-1 against human hepatocellular liver carcinoma (HepG2) cells to investigate t...

The nonconventional yeast Yarrowia lipolytica degrades very efficiently hydrophobic substrates to produce organic acids, single-cell oil, lipases, and so forth. The aim of this study was to investigate the biochemical behavior and simultaneous production of valuable metabolites such as lipase, citric acid (CA), and single-cell protein (SCP) by Yarrowia lipolytica DSM 3286 grown on various plant...

This research is a nutritional assessment of a fungal (Aspergillus fumigatus 1-21A) single-cell protein (SCP) that was fed to rats and pigs. The sun-dried biomass resulted from fermentation of either fresh roots or cassava meal substrates and had the following percentage composition: crude protein (N x 6.25), 34.3; ether extract, 3.1; crude fiber, 20.0 ash, 4.3; N-free extract, 27.9; Ca, .20 an...

Abbreviations: BOD: biochemical oxygen demand COD: chemical oxygen demand OD: optical density OW: optimized wastewater PNSB: purple non sulphur photosynthetic bacteria ROW:raw optimized wastewater RW: raw wastewater SCP: single cell protein SEM: scanning electron microscope SOW: sterile optimized wastewater SW: sterile wastewater TEM: transmission electron microscope TKN: total kjeldahl nitroge...

Single-cell protein (SCP) derived from methanotrophic bacteria has significant potential as a fishmeal alternative in aquafeeds. However, SCP known palatability issues, to overcome these issues tuna hydrolysate and garlic powder were tested enhancers against basal diets without additives. This study the inclusion of at four dietary levels 0%, 10%, 20% 30%, representing 25%, 50% 75% replacement ...

Abstract Large-scale production of single-cell protein (SCP) has the potential not only to solve some food insecurity and water scarcity crises that plague a significant portion our world today but also holds promise reduce cost associated with treatment industrial agricultural wastewater. Resource recovery SCP from organic waste by microbes like yeast microalgae is commonly documented. However...

In this study, twenty-five whey samples collected from dairy industries in the city of Isfahan. The sampleswere cultured on malt extract broth (MEB) and yeast extract glucose chloramphenicol agar (YGCA) media.Eleven yeast strains (designated M1 to M11) were isolated from the culture. The strains were identified bytheir morphological and physiological properties. Betagalactosid...

Single-cell transcriptome sequencing highly requires a convenient and reliable method to rapidly isolate a live cell into a specific container such as a PCR tube. Here, we report a modular single-cell pipette (mSCP) consisting of three modular components, a SCP-Tip, an air-displacement pipette (ADP), and ADP-Tips, that can be easily assembled, disassembled, and reassembled. By assembling the SC...

A major gap in our knowledge of rapid hepatic HDL cholesterol clearance is the role of key intracellular factors that influence this process. Although the reverse cholesterol transport pathway targets HDL to the liver for net elimination of free cholesterol from the body, molecular details governing cholesterol uptake into hepatocytes are not completely understood. Therefore, the effects of ste...