Spent coffee grounds (SCG) are the main residues from the soluble coffee industry. The objective of this work was to evaluate different extraction methods of oil and diterpenes (kahweol and cafestol) from SCG, such as solid-liquid extraction, supercritical extraction or direct saponification, as well as to determine the phenolic composition and antioxidant capacities SCG and defatted SCG. Among...

Application of a recently developed phospholipid saponification proce dure to malignant colon or breast tissue produces an unidentified pliosphodiester resonance in the •¿ "!' nuclear magnetic resonance spectrum which appears to correlate with malignancy. Glycerol phosphodiesters were prepared from a malignant breast tumor by a Folch extraction of the tissue followed by saponification of the ...

Biomarkers are essential for assessment of oxidative stress and evaluation of antioxidant capacity in vivo. Total hydroxyoctadecadienoic acid (HODE)and7-hydroxycholesterol measured afterreduction and saponification ofbiological fluidsmaybeusedasreliablebiomarker. J. Med. Invest. 52 Suppl. : 228-230, November, 2005

A general mechanism underlying the response of ectotherms to environmental changes often involves changes in fatty acid composition. Theory predicts that a decrease in temperature causes an increase in unsaturation of fatty acids, with an important role for long-chain poly-unsaturated fatty acids (PUFAs). However, PUFAs are particularly unstable and susceptible to peroxidation, hence subtle dif...