Seven-thousand-and-thirty-six specimens of faeces were examined for Salmonellae using three enrichment culture media in parallel.Rappaport's magnesium chloride malachite green broth medium proved superior to selenite F and tetrathionate broths in the recovery of a wide variety of Salmonella serotypes. However, it was inferior to selenite broth in the recovery of Salmonella typhi.A higher recove...

One hundred seventy-five samples of fresh pork sausage representing thirty-five different commercial brands from six different retail stores were examined for the presence of salmonellae by standard enrichment, plating, biochemical and serological techniques. Contamination levels varied from 0 to 50% among stores and from 0 to 28% among brands. Prior research implied reduced prevalence of salmo...

The competitive exclusion (CE) action of Aviguard (AG) and its effects on the antibody response of chicks were evaluated in this study. We observed that AG protected the chicks from overwhelming colonization. Fourteen days after infection, fewer AG-pretreated than nonpretreated chicks shed salmonellae from their coloaca in both infected groups, although much less from SE-infected chicks. Antibo...

Two thousand four hundred and ninety eggs were collected from retail markets in 6 provinces and from laying hen farms in 3 provinces. Eggs were pooled in groups of 3 to obtain 830 samples for testing. Isolation of salmonellae was made from both egg shell and egg contents. Eggs from retail markets were contaminated with salmonellae on egg shells (13.2%) and in egg contents (3.9%). Three (0.4%) s...

Salmonellae are facultative intracellular bacteria capable of surviving within macrophages. Salmonella pathogenicity island 2 (SPI-2) is required for growth within macrophages and for virulence in mice. In this study, we show the involvement of SPI-2 in a signal transduction pathway that induces cytokine expression in Salmonella-infected macrophages. High levels of interleukin-10 (IL-10) mRNA w...

The present research work was conducted to study about the seroprevalence and pathology of Salmonella infections in layer chickens of Dhaka and Gazipur regions of Bangladesh and to isolate and characterize Salmonellae from layer chickens during the period from January to May 2006. The used materials were blood sample, cloacal and liver swabs from live and dead birds respectively, and visceral o...

Contamination of seafood with salmonellae is a major public health concern. Detection of Salmonella by standard culture methods is time consuming. In this study, an enrichment culture step prior to polymerase chain reaction (PCR) was applied to detect 284 bp fragment of Salmonella invA in comparison with the conventional culture method in 100 shrimp samples collected from four different shrimp ...

The mode of antimicrobial action of chitosan (polymeric beta-1,4-N-acetylglucosamine) on gram-negative bacteria was studied with special emphasis on its ability to bind to and weaken the barrier function of the outer membrane (OM). Chitosan (250 ppm) at pH 5.3 induced significant uptake of the hydrophobic probe 1-N-phenylnaphthylamine (NPN) in Escherichia coli, Pseudomonas aeruginosa and Salmon...

Most strains of Salmonellae have simple nutritional requirements and grow well, therefore, in simple media consisting of glucose, inorganic nitrogen, and mineral salts (Kauffmann, 1954). There are, however, a number of Salmonellae which are unable to synthesize certain essential vitamins and amino acids and these must be supplied in the culture medium. The need of Salmonella typhosa for tryptop...

Several investigators have studied cold-blooded animals in terms of contamination by different types o f organisms. Hinshaw and M cN eil4 isolated Salm o­ nella rubislaw from 3 out o f 12 Sceloporus occi­ d e n t a l specimens. Zwart24 reported the iso­ lation o f Salmonellae from 37.5% of lizards. LeN oc and Brygoo9 isolated 58 different types o f sal­ monellae from 48 out o f 127 chameleons e...